Protective effects of vitamin C against Cyclophosphamide-Induced Ovarian Toxicity in mice.

Background: The side effects of Cyclophosphamide causes reduction of fertility or even infertility in patients treated with this drug. For this reason, the present study has conducted to investigate the protective different effects of vitamin C on ovarian toxicity of Cyclophosphamide in mice.Methods: In this experimental study, 28 adult female mice of 4 months old from the race balb / c, in the weight range of 50-40 g randomly divided into 4 groups of 7. For the mice of control group 1 dose Saline (IP, 75mg / kg), to the group 2 daily for 7 days Vitamin C (IP, 1000 mg/kg), to the group 3 one dose of Cyclophosphamide (IP, 75mg / kg) and for the mice of group 4 vitamin C in the same way with the injection of a dose of Cyclophosphamide (IP, 75mg / kg) was prescribed. For the 14th day of test (after one cycle of ovulation), animals were euthanized and the right ovary was used for histological studies and morphometrics. Analysis of data was performed by using one-way ANOVA and Tukey post hoc test. The significant level of P 0/05 was considered.Results: Cyclophosphamide resulted in a significant decrease in the number of ovarian follicles and in the number of Corpus luteum compared with the control group (P 0/05). Prescription of vitamin C along with Cyclophosphamide had a protective effect on the ovary, so that the number of ovarian follicles and corpus luteum was greater than the same amountsin the Cyclophosphamide group. Of course, among the evaluated parameters, only the number of preliminary follicles was significant (P 0/05).Conclusion: According to the results of this study, prescription of vitamin C can significantly prevent the negative effects resulting from prescription of Cyclophosphamide on the ovarian tissue,