پديدآورندگان :
Hamidian Gh Department of Basic Science, , Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran , Hanifeh M Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran , Jarolmasjed S.H Department of Clinical Science, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran , Fadaei, A.M Department of Basic Science, , Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran , Azadian A a.azadian90@ms.tabrizu.ac.ir Department of Basic Science, , Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
كليدواژه :
Erythropoietin , Liver , Kupffer cells , Ischemia , reperfusion , Cat , Stereology
چكيده فارسي :
Objective: The liver is one of the largest resident populations of macrophages or Kupffer cells (KC) which are key components of the innate immune system. These cells are intimately involved in the liver’s response to transient ischemia and various other stresses. Despite many published studies reporting the beneficial effects of pharmacological preconditioning with EPO on reperfusion injury of various tissues, but there are no information about the hepatoprotective role of EPO following intestinal ischemia-reperfusion (I/R) in cat.Materials Methods: In this study, 20 tomcats were divided into 4 groups including: 1) sham which had laparatomy operation; 2) jejunum I/R (60/60 minutes) which received normal saline; 3) jejunum I/R which received 1000 IU/kg EPO and 4) jejunum I/R which received 3000 IU/kg EPO via IV after ischemia and before reperfusion. One hour after reperfusion, Liver removed and fixed in in 10% neutral buffered formalin. The samples were processed by routine and standard paraffin embedding and serially sections stained by H E. For estimating of Kupffer cells (KC) number, the sampling of physical dissector pairs was obtained based on uniform systematic random strategy and numerical density of KC and liver volume was estimated using an unbiased counting frame and Cavalieri s principle. One-way ANOVA followed by the Tukey post hoc performed for data analysis. A P-value of less than 0.05 was considered significant.Results Conclusion: Stereological results showed that ischemia-reperfusion in the jejunum could significantly increase population of KC compared to other group (p 0.05). Data analysis also indicated that erythropoietin could control increasing of KC population in a dose dependent manner (p 0.05). Results revealed that high dose of erythropoietin had a better effect than low dose of it. It can be concluded that administration of erythropoietin can improve side effect of jejunum ischemia-reperfiusion on the histopathological features of the liver in cat.
