Study the Metal (Ag) and Metal oxide (TiO2 and ZnO) nanoparticles Interaction with Enzymatic detergents: Amylase, Cellulase and Lipase

Nanoparticles have special properties due to their size, as their size decreases their surface energy increases, which causes higher reactivity. Metal (Ag) and metal oxide (TiO2 and ZnO) nanoparticles are applicable in different fields of industry; healthcare and agriculture caused by biochemical properties including antimicrobial or photo-degradation activity. One of the applications of mentioned nanoparticle is their deposition on clothes and textiles for their self-cleaning, shining and antibacterial activity. Moreover, different types of enzymes are important factors in the development of cleaning products, such as laundry detergents and dishwashing liquids. Utilizing enzyme detergents is more efficient, makes the washing environment milder and saves more energy. Modified textiles are being in contact with enzymes used in laundry detergents including amylase, cellulase and lipase while washing process, causing the loss of original properties of enzymes. The aim of this project is to investigate the interaction of metal (Ag) and metal oxide (TiO2 and ZnO) nanoparticles and amylase, cellulase and lipase as detergent enzymes. In this case parameters influenced on their features including nanoparticle-enzyme ratio, time and temperature were monitored using central composite design (CCD). Finally the physiochemical and biochemical features and variations of enzymes as well as nanoparticles were investigated based on enzyme assay and model bacteria (model microorganisms including Staphylococcus aureus and Escherichia coli) survive. The activity of enzyme was studied using water contact angle and the nanoparticles physiochemical features variations were monitored using UV-Vis, CD and DLS.