Antioxidant Investigation of Marjoram using Aminated Carbon Nanoparticles/Glassy Carbon Modified Electrode

Development of new methods for determination of antioxidants in natural compounds has received considerable interests in recent years. Various methods such as chromatography, spectrophotometry and capillary electrophoresis (CE) have been developed for this purpose. These techniques are limited by poor accuracy or the need for expensive instrumentation with high operating costs. Among the various reported methods, electrochemical techniques are often preferred because of their high selectivity, rapid detection, low cost and their lack of a substantial sample preparation setup [1]. Marjoram belongs to the mint family. Flowers and leaves are the parts of the plant are used in medicinal preparations. Marjoram is packed with vitamins and minerals, that gives the following health related properties: anti-microbial, anti-septic, antiinflammatory, anti-depressant, anti-viral and antioxidant. Gallic acid is a natural antioxidant that not synthesized in the human body [2]. It has reported that the main compound responsible for antioxidant properties of Marjoram is Gallic acid. In this study, glassy carbon electrode has been modified by using Alginate/CNP-NH3 + bilayer. The formation of bilayer is based on electrostatic interaction between two components. The modified electrode has been studied using cyclic voltammetry, electrochemical impedance spectroscopy (ESI) and scanning electron microscopy (SEM). The modified electrode has been employed for determination of gallic acid. The gallic acid has been used as standard for antioxidant properties of Marjoram.