Voltammetric sensor based on a new synthesized ligand and Mn3O4/SnO2 nanoparticles MCPE for determination of sumatriptan in biological fluids.

Sumatriptan succinate is a 5-HT1 receptor agonist which is used in the treatment of migraine. It shows low bioavailability (15%) due to high hepatic first pass metabolism (1). SUM reduces the vascular in flammation associated with migraines. However, an over dose is toxic and leads to several side effects viz. , paresthesia, warm/cold sensations, chest pain, fatigue and vertigo. Thus, its determination is of importance (2). In this study, a new Schiff base ligand, N ,2-bis(2-hydroxybenzylidene)hydrazine-1- carbothiohydrazide (figure 1) , has been synthesized by reaction of the hydrazinecarbothiohydrazide with 2-hydroxybenzaldehyde at ethanol under mixture stirred at room temperature for 30 Minute. The structure of the synthesized compound resulted from the IR and 1H NMR. Afterwards, a carbon paste electrode modified with nanocatalyste (Mn3O4/SnO2) and a new thiohydrazin derivative Schiff base was developed for the sumatriptan determination at nanomolar level concentration. The electrochemical behavior of this molecule was investigated employing cyclic voltammetry and anodic stripping voltammetric. The experiments were conducted with a certain electrode composition of 18.0% paraffin oil, 69.0% high purity graphite powder, 6% NiFe2O4 and 6% Shiff base as well as the usage of 0.2 M phosphate buffer as supporting electrolyte. Also the enhanced peak current response is a clear evidence of the catalytic of the modified carbon paste electrode towards oxidation of sumatriptan. The effect of pH and scan rate on the electrode process was investigated. The resulting electrode demonstrated linear response across a wide sumatriptan concentration range with a detection limit value of 10-8 M, on the basis of a signal to noise ratio of 3. The method was successfully applied to the determination of sumatriptan in pharmaceutical tablets and plasma.