Evaluation of chemical composition,secondary metabolites (phenol,flavonoid),antioxidant effect and biological activities of Golden rain leaves

Evaluation of chemical composition,secondary metabolites (phenol,flavonoid),antioxidant effect and biological activities of Golden rain leaves

This study evaluates the antioxidant and antimicrobial activities of methanolic extract of Golden rain leaves on various plant pathogens that commonly cause irreparable damages to agricultural crops. Also, total phenolic and flavonoid content was determined. Antimicrobial activity was tested against 14 microorganisms, including three gram-positive (Bacillus subtilis, Staphylococcus aureus and Rathayibacter toxicus), five gram-negative (Escherichia coli, Pseudomonas aeruginosa, Pseudomonas syringae subsp. syringae, Pseudomonas viridiflava and Xanthomonas campestris pv. campestris) bacteria and six (Pyricularia grisea, Fsarium oxysporum, Sclerotinia sclerotiorum, Botrytis cinerea, Rhizoctonia solani and Alternaria alternata) fungi, using disk diffusion method and the Minimum Inhibitory Concentration (MIC) technique. The Golden rain leaves were also subjected to screening for possible antioxidant activity by using catalase, peroxidase, superoxide dismutase and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The methanolic extract showed significant activity against S. aureus (MIC = 25 µg/mL), and P. oryzae (MIC = 12.5 µg/mL). In addition, the free radical scavenging capacity of the extract was determined with an IC50 value of 113.17 µg/mL. Furthermore, structure of the compounds was determined by means of Liquid chromatography with UV and MS detection (LC/UV/ESI-MS). Using this method of analysis, we detected up to three saponin and acyclic sesquiterpene oligoglycoside. The plant produces these compounds as a complex mixture of naturally non-regiosselective acetylated glycosides. Our results suggest that this plant may be a potential source of biocide, for economical and environmentally friendly disease control strategies. It may also be a good candidate for further biological and pharmacological investigations.

This study evaluates the antioxidant and antimicrobial activities of methanolic extract of Golden rain leaves on various plant pathogens that commonly cause irreparable damages to agricultural crops. Also, total phenolic and flavonoid content was determined. Antimicrobial activity was tested against 14 microorganisms, including three gram-positive (Bacillus subtilis, Staphylococcus aureus and Rathayibacter toxicus), five gram-negative (Escherichia coli, Pseudomonas aeruginosa, Pseudomonas syringae subsp. syringae, Pseudomonas viridiflava and Xanthomonas campestris pv. campestris) bacteria and six (Pyricularia grisea, Fsarium oxysporum, Sclerotinia sclerotiorum, Botrytis cinerea, Rhizoctonia solani and Alternaria alternata) fungi, using disk diffusion method and the Minimum Inhibitory Concentration (MIC) technique. The Golden rain leaves were also subjected to screening for possible antioxidant activity by using catalase, peroxidase, superoxide dismutase and 2, 2-diphenyl-1-picrylhydrazyl (DPPH) assay. The methanolic extract showed significant activity against S. aureus (MIC = 25 µg/mL), and P. oryzae (MIC = 12.5 µg/mL). In addition, the free radical scavenging capacity of the extract was determined with an IC50 value of 113.17 µg/mL. Furthermore, structure of the compounds was determined by means of Liquid chromatography with UV and MS detection (LC/UV/ESI-MS). Using this method of analysis, we detected up to three saponin and acyclic sesquiterpene oligoglycoside. The plant produces these compounds as a complex mixture of naturally non-regiosselective acetylated glycosides. Our results suggest that this plant may be a potential source of biocide, for economical and environmentally friendly disease control strategies. It may also be a good candidate for further biological and pharmacological investigations.