چكيده فارسي :
Tartrazine, also known as Acid Yellow 23 or the additive E102 provides a vivid yellow color to the commercial beverages and foods as well as drugs and personal care products [1]. Tartrazine, has a sulfonic group as an auto chrome that makes it highly water soluble and polar. It is reported that Tartrazine is often responsible for allergic reactions, and, it is suspected to be carcinogenic and mutagenic due to its conversion into the aromatic aminesulphanilic acid in metabolic reactions [2].
The enzyme laccase (polyphenoloxidase; EC 1.10.3.2) is a member of the blue multi-copper-oxidase family. These enzymes have been studied for a long time, due to their ability to oxidize a variety of organic substrates, and to reduce molecular oxygen to water [3]. Azo dye degradation by laccases starts by asymmetric cleavage of the azo bond followed by oxidative cleavage, desulfonation, deamination, demethylation and dihydroxylation, depending on dye structure [4].
Monitoring of Tartrazine in the food industry, environmental and bio-medical analyses, by using portable, cost effective devices, has become an area of growing interest over the past decade
A new Laccase amprometric biosensor based on Fe3O4/Graphene Oxide/Chitosan nanocomposite for determination of Tartrazine(TZ) was described. The nanocomposite was electrodeposited on the surface of screen printed carbon electrode (SPCE) by one-step electrodeposition of the nanocomposite at an applied potential of -2 V (vs. Ag/AgCl) for 60 s. For biosensor preparation, modified SPCE was immersed in the solution containing glutaraldehyde (2.5%) as cross-linker for 2 h, enabling the formation of a covalent bond between the aldehyde group of glutaraldehyde and the amine group of the protein (Laccase). Filed effect Scanning electron microscopy (FE-SEM), Fourier transform infrared (FTIR)
13th Annual Electrochemistry Seminar of Iran
Materials and Energy Research Center (MERC), 22- 23 Nov, 2017
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spectrophotometry, cyclic voltammetry (CV), and electrochemical impedance spectroscopy (EIS) were used for characterization of the electrode surface.
The biosensor was optimized with respect to biocomposite composition, enzyme loading, and solution pH by amperometry method the applied potential of –0.25 V (vs. Ag/AgCl. The biosensor exhibited noticeable eletrocatalytic ability toward tartrazine with a linear concentration range from 0.03 to 10 μg/L and a detection limit of 10 ng /L. The fabricated biosensor was successfully applied for measurement of Tartrazine in soft drinks.
