Adsorptive potentiometric stripping analysis of anticancer drug at different electrodes and different electrolytes.

In this work, effect of different electrodes such as glassy carbon (GC), Au, Pt, MWCNT past and MWCNT-GC electrodes and different electrolytes such as HNO3, CH3PO4, CH3COOH and B-R buffer on the electrochemical behavior of Tamoxifen (Tam) as breast anticancer drug were investigated by differential-pulse anodic adsorptive striping (DPAAS) voltammetric techniques. It was observed that at GCE and H2SO4 as electrolyte, electrocatalytic behavior for the oxidation of Tam was excellent. It was evidenced by the enhancement of oxidation peak current and shift in the oxidation potential to less positive values. Cyclic voltammetry (CV) and chronoamperometry were used to understand the electrochemical characteristics of Tam. A chronoamprograms gave fundamental electrochemical parameter including the electroactive surface coverage (Ʈ), the diffusion coefficient (D) and the heterogeneous rate constant (ks). Based on the results of the recorded CV, the electrodeposition and anodic striping behavior of the Tam were investigated at the surface of GCE. The primary experiments demonstrated that the DPAASV presents a sufficient oxidation peak current at approximately 1.03 V vs Ag/AgCl. Therefore, to find the best conditions for taking a sharp analytical peak concerning the electro-oxidation of Tam, the effects of different factors such as scan rate, deposition potential and deposition time on anodic peak have been studied and optimized. The calibration curve showed linearity in the 13th Annual Electrochemistry Seminar of Iran Materials and Energy Research Center (MERC), 22- 23 Nov, 2017 100 range of 0.5 to 80 μM and the limits of detection (LOD) and quantitation (LOQ) were calculated to be 0.12 and 0.4 μM, respectively. The mean, standard error and relative standard deviation (RSD) for 4 replicates of 15 μM were found to be 15.57 μM, 3% and 4%, respectively. Finally, it has been successfully applied to the determination of Tam in different spiked physiological samples.