ﻣﻄﺎﻟﻌﻪ اﺛﺮات ﻓﻨﺎﻧﺘﺮن ﺑﺮ ﺳﺎﺧﺘﺎر ﺑﺎﻓﺖ ﮔﻨﺎدي ﻣﺎﻫﯽ ﺷﺎﻧﮏ زردﺑﺎﻟﻪ (Acanthopagrus latus) ﻧﺎﺑﺎﻟﻎ

Study of the Effects of Phenanthrene on Gonadal Tissue Structure of Immature Acanthopagrus Latus

اﯾﻦ ﭘﮋوﻫﺶ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳـــﯽ اﺛﺮات ﻓﻨﺎﻧﺘﺮن ﮐﻪ ﯾﮑﯽ از ﺗﺮﮐﯿﺒﺎت ﻣﻬﻢ ﻫﯿﺪروﮐﺮﺑﻦﻫﺎي آروﻣﺎﺗﯿﮏ ﭼﻨﺪ ﺣﻠﻘﻪاي (PAH) ﻣﯽﺑﺎﺷﺪ ﺑﺮ ﺳﺎﺧﺘﺎر ﺑﺎﻓﺖ ﮔﻨﺎدي ﻣﺎﻫﯽ ﺷﺎﻧﮏ زردﺑﺎﻟﻪ (Acanthopagrus latus )ﻧﺎﺑﺎﻟﻎ ﺻﻮرت ﮔﺮﻓﺖ. ﺑﺪﯾﻦ ﻣﻨﻈﻮر ﺟﻬﺖ ﺑﺮرﺳﯽ اﺛﺮات ﮐﻮﺗﺎه ﻣﺪت و ﺑﻠﻨﺪ ﻣﺪت ﻓﻨﺎﻧﺘﺮن ﺑﺮ اﯾﻦ ﮔﻮﻧﻪ، ﻣﺎﻫﯿﺎن ﺑﻪ 6 ﮔﺮوه ﺷــﺎﻣﻞ ﺷــﺎﻫﺪ، ﮐﻨﺘﺮل ﺣﻼل و ﮔﺮوهﻫﺎي ﺗﯿﻤﺎر ﺑﺎ ﻏﻠﻈﺖﻫﺎي ﻣﺨﺘﻠﻒ ﻨﺎﻧﺘﺮن ﺗﻘ ﺴﯿﻢ و ﻃﯽ دوره 14 روزه ﻣﻮرد ﻣﻄﺎﻟﻌﻪ ﻗﺮار ﮔﺮﻓﺘﻨﺪ. ﻃﺒﻖ ﻧﺘﺎﯾﺞ ﺑﻪ د ﺳﺖ آﻣﺪه ﻣﯿﺰان ﺗﺠﻤﻊ ﻓﻨﺎﻧﺘﺮن در ﮐﻞ دوره در ﮔﺮوه ﺷﺎﻫﺪ و ﮐﻨﺘﺮل ﺣﻼل ﺑﺮاﺑﺮ ﺑﺎ ﺻﻔﺮ ﺑﻮد و در روز اول ﻧﯿﺰ ﻣﯿﺰان ﺗﺠﻤﻊ ﭘﺎﯾﯿﻦﺗﺮ از ﺣﺪ ﺗﺸﺨﯿﺺ دﺳﺘﮕﺎه ﺑﻮد. ﺑﯿﺶﺗﺮﯾﻦ ﻣﯿﺰان ﺗﺠﻤﻊ در ﺑﺎﻓﺖ ﮔﻨﺎدي در روز ﭼﻬﺎرم و در ﺑﯿﺶﺗﺮﯾﻦ ﻏﻠﻈﺖ ﻓﻨﺎﻧﺘﺮن ﺑﺎ ﻣﯿﺰان 70 ﻣﯿﮑﺮوﮔﺮم ﺑﺮ ﮐﯿﻠﻮﮔﺮم ﺑﻪ دﺳـــﺖ آﻣﺪه ﮐﻪ ﺗﺠﻤﻊ آن در ﺑﺎﻓﺖ 2/69 ﻧﺎﻧﻮﮔﺮم ﺑﺮ ﮔﺮم وزن ﺧﺸﮏ ﺳﻨﺠﺶ ﮔﺮدﯾﺪ. ﻃﺒﻖ ﺑﺮرﺳﯽ ﻫﯿﺴﺘﻮﭘﺎﺗﻮﻟﻮژﯾﮑﯽ ﺑﺎﻓﺖ ﮔﻨﺎدي ﻫﯿﭻ ﮔﻮﻧﻪ ﺿﺎﯾﻌﺎت ﺑﺎﻓﺘﯽ ﭼﻪ در ﮔﺮوه ﺷﺎﻫﺪ و ﮐﻨﺘﺮل ﺣﻼل و ﭼﻪ در ﮔﺮوهﻫﺎي ﺗﯿﻤﺎر ﻧﺸﺎن ﻧﺪاد.

This study was carried out to evaluate the effects of phenanthrene, an important component of polycyclic aromatic hydrocarbons (PAH), on the structure of gonadal tissue of immature blueberries (Acanthopagrus latus). For this purpose, in order to investigate the short-term and long-term effects of phenanthrene fishes, they were divided into 6 groups including control, solvent control and treatment groups with different concentrations of phenanthrene and studied for 14 days. According to the results, the accumulation of phenanthrene in the whole period was equal to the control group and the control of the solvent, and on the first day, the accumulation rate was lower than the detection rate of the device. The highest accumulation in gonadal tissue was obtained on day 4 and at the highest concentrations of phenanthrene 70 μg / kg, which accumulated in tissue of 2.69 ng/g dw. According to the histopathologic examination of gonadal tissue, no tissue lesions were observed in the control and control groups, or in the treatment groups.