چكيده فارسي :
ﻫﺪف از اﻧﺠﺎم اﯾﻦ ﺗﺤﻘﯿﻖ ﺑﺮرﺳﯽ ﻓﺎﮐﺘﻮرﻫﺎي ﺧﻮﻧﯽ و ﺳﺮم ﺧﻮن دو زﯾﺮﮔﻮﻧﻪ ﺷﮓ ﻣﺎﻫﯽ (A. ، A. brashnikovi kisselevitshi brashnikovi orientalis)ﻣﯽﺑﺎﺷﺪ. در ﻫﻤﯿﻦ راﺳﺘﺎ ﺗﻌﺪاد 30 ﻗﻄﻌﻪ ﻣﺎﻫﯽ ﺗﻮﺳﻂ ﺷﺮﮐﺖﻫﺎي ﺗﻌﺎوﻧﯽ ﭘﺮه ﻃﯽ ﻓﺼﻞ ﺻﯿﺪ ﺳﺎل 94-1393 از آﺑﻬﺎي ﺟﻨﻮب ﺷﺮﻗﯽ درﯾﺎي ﺧﺰر ﺻﯿﺪ ﺷﺪ. ﻧﻤﻮﻧﻪﻫﺎي ﺻﯿﺪ ﺷﺪه ﭘﺲ از ﺳﻨﺠﺶ وزن و ﻃﻮل، از ﻗﺴﻤﺖ ﺳﺎﻗﻪ دﻣﯽ ﺧﻮﻧﮕﯿﺮي ﺷﺪﻧﺪ. ﺳﭙﺲ ﺷﺎﺧﺺﻫﺎي ﺧﻮن ﺷﻨﺎﺳﯽ (, Lym, Mono, Neut, Eos) و ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ (ﮔﻠﻮﮐﺰ، ﺗﺮيﮔﻠﯿﺴﯿﺮﯾﺪ، ﮐﻠﺴﯿﻢ، ﮐﻠﺴﺘﺮول، آﺳﭙﺎرﺗﺎت آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز، آﻻﻣﯿﻦ آﻣﯿﻨﻮﺗﺮاﻧﺴﻔﺮاز، آﻟﮑﺎﻟﯿﻦ ﻓﺴﻔﺎﺗﺎز، ﻟﮑﺘﺎت دﻫﯿﺪروژﻧﺎز) ﺑﺎ روشﻫﺎي اﺳﺘﺎﻧﺪارد آزﻣﺎﯾﺸﮕﺎﻫﯽ ﻣﻮرد ﺳﻨﺠﺶ ﻗﺮار ﮔﺮﻓﺖ. ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از ﺑﺮرﺳﯽ ﺷﺎﺧﺺﻫﺎي ﺧﻮن ﺷﻨﺎﺳﯽ و ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ دو زﯾﺮﮔﻮﻧﻪ ﺷﮓ ﻣﺎﻫﯽ ﻧﺸﺎن داد ﮐﻪ ﺑﯿﺸﺘﺮﯾﻦ ﻣﯿﺰان ﺷﺎﺧﺺﻫﺎي ﮔﻠﺒﻮل ﺳﻔﯿﺪ، ﻫﻤﻮﮔﻠﻮﺑﯿﻦ، ﻫﻤﺎﺗﻮﮐﺮﯾﺖ و ﻏﻠﻈﺖ ﻣﺘﻮﺳﻂ ﻫﻤﻮﮔﻠﻮﺑﯿﻦ ﮔﻠﺒﻮلﻫﺎي ﻗﺮﻣﺰ در A. b. orientalis اﻧﺪازه ﮔﯿﺮي ﺷﺪ و اﯾﻦ ﺗﻔﺎوت از ﻧﻈﺮ آﻣﺎري ﻣﻌﻨﯽ دار ﺑﻮد. در ﺳﺎﯾﺮ ﺷﺎﺧﺺﻫﺎي ﻣﻮرد ﺑﺮرﺳﯽ ﺑﯿﻦ دو زﯾﺮﮔﻮﻧﻪ اﺧﺘﻼف ﻣﻌﻨﯽ داري از ﻧﻈﺮ آﻣﺎري ﻣﺸﺎﻫﺪه ﻧﺸﺪ. ﻧﺘﺎﯾﺞ ﺣﺎﺻﻞ از ﺑﺮرﺳﯽ ﻣﯿﺰان ﺷﺎﺧﺺﻫﺎي ﺧﻮن ﺷﻨﺎﺳﯽ، دو ﮔﻮﻧﻪ ﺷﮓ ﻣﺎﻫﯽ ﻧﺸﺎن ﻣﯽدﻫﺪ اﺧﺘﻼف در ﺑﺮﺧﯽ ﺷﺎﺧﺺﻫﺎي ﺧﻮﻧﯽ ﻣﯽﺗﻮاﻧﺪ ﺗﺤﺖ ﺗﺎﺛﯿﺮ ﻓﻌﺎﻟﯿﺖﻫﺎي ﻓﯿﺰﯾﻮﻟﻮژﯾﮏ و اﺳﺘﺮسﻫﺎي ﻣﺤﯿﻄﯽ ﺑﺎﺷﺪ ﮐﻪ ﻣﺎﻫﯿﺎن ﻧﺴﺒﺖ ﺑﻪ اﯾﻦ ﻋﻮاﻣﻞ ﺑﺴﯿﺎر ﺣﺴﺎس ﻫﺴﺘﻨﺪ.
چكيده لاتين :
The aim of this study was to evaluate serum parameters five subspecies of Clupidae, Alosa sp. (A. brashnikovi kisselevitshi and A. brashnikovi orientalis). Samples were collected from Southwest Caspian Sea coastlines in spring 2015. After biometry, fish blood samples were obtained from the caudal vein. Blood factors with standard laboratory methods was evaluated. Comparison of means of hematological (RBC, WBC, MCV, MCH, MCHC, Hb, PCV, Lym, Mono, Neut, Eos) and biochemical (Glucose, triglyceride, calcium, cholesterol, aspartate aminotransferase, alamine aminotransferase, alkaline phosphatase, lactate dehydrogenase) parameters measured by standard laboratory methods. The results revealed the highest levels was measured of WBC, Hb, PCV, MCHC in A. b. orientalis and this difference was statistically significant. In other parameters between the two subspecies this difference was not statistically significant. The results of hematological factors show differences in some of the blood parameters can be influenced by physiological activities and environmental stresses, that fish are very sensitive to these factors.
