شماره ركورد كنفرانس :
4518
عنوان مقاله :
Preparation, Characterization and Application of Fe3O4 Clusters@nSiO2@mSiO2 Nanocomposite Particles for Lipase Immobilization
Author/Authors :
Mohammad Kalantari Department of Industrial and Environmental Biotechnology - National Institute of Genetic Engineering and Biotechnology , Ayyoob Arpanaei Department of Industrial and Environmental Biotechnology - National Institute of Genetic Engineering and Biotechnology , Mohammad Kazemeini Department of Chemical and Petroleum Engineering - Sharif University of Technology , Fatemeh Tabandeh Department of Industrial and Environmental Biotechnology - National Institute of Genetic Engineering and Biotechnology
كليدواژه :
Immobilization , Lipase , Magnetite Nanoparticles , Nonporous Silica , Porous Silica
عنوان كنفرانس :
The 7th International Chemical Engineering Congress & Exhibition (IChEC 2011
چكيده لاتين :
Multifunctional Fe3O4 clusters/nonporous SiO2/mesoporous SiO2 (core/shell/shell) nanocomposite particles were prepared in order to develop a lipase-based recyclable nanobiocatalyst used in the biofuel production. Magnetite nanoparticles were synthesized by coprecipitation method and their clusters were coated by a silica thin shell using the modified Stöber method. Through a template removing approach, a mesoporous silica shell was formed on the nonporous silica. Calcination method in the presence of complex salts was used to expand the pore size of nanoparticles. The obtained nanoparticles possess superparamagnetism, high magnetization, high mean pore diameter (28.5 nm), high surface area (26 m2/g), and large pore volume (0.187 cm3/g). Once nanocomposite particles were prepared, lipase molecules were covalently immobilized onto nanocomposite particles by using glutaraldehyde as an activating agent. Finally, the loading and activity of the immobilized lipase were measured.
