G3BP1 and VEZATIN Gene Expression in gastric cancer Patients

Gastric cancer in the world and the third leading cause of cancer-related death in both sexes worldwide. Overexpression of G3BP1 protein in several types of human tumors such as gastric cancer. VEZT gene encodes an adherence junctions’ transmembrane protein called VEZATIN. VEZATIN has three domains, extracellular, transmembrane, and intracellular. The objective of this study was to examine the gene expression profiles of G3BP1 and VEZATIN in Iranian Gastric cancer patients. Expression of these genes in FFPE samples collected from three groups of participants was analyzed. The first group consisted of 40 patients whose Gastric Cancer. The second group included 40 normal samples. Differences in gene expression among metastatic and normal samples were estimated using Student GraphPad Prism 5 with 1way ANOVA analysis. Using the GelQuantNET software for analysis of Real time PCR products. The relative gene expression levels were determined using the (∆∆ct) method. Parameters considered included ER-positive, lymph node negative, tumor size less than 5 cm in diameter. RNA extraction and cDNA synthesis were performed by using ABI FFPE kit. Analysis of gene expression using real-time PCR revealed that the relative abundance of G3BP1 transcripts was up-regulated and that of VEZATIN transcripts was down in the metastatic group compared with that of normal samples. The results suggest that gene expression profiling can be used for predicting the recurrence of Gastric Cancer and the expression levels of G3BP1, VEZATIN could serve as a prognostic biomarker in Gastric Cancer recurrence.