مقايسه فعاليت ﺳﯿﺘﻮﺗﻮﮐﺴﯿﮏ ﻋﺼﺎره هاي (شلاق دريا) از خانواده Ellisellidae در خليج فارس

Comparison of cytotoxic activity of extracts of two soft coral species from Ellisellidae family in the Persian Gulf

تركيبات ﺳﯿﺘﻮﺗﻮﮐﺴﯿﮏ ﻋﻮاﻣﻠﯽ ﻫﺴﺘﻨﺪ ﮐﻪ ﺑﻪ ﻃﻮر ﻣﺴﺘﻘﯿﻢ ﺳﻠﻮل را ﻧﺎﺑﻮد ﻣﯽ ﻧﻤﺎﯾﻨﺪ. دراين تحقيق مرجان ها به روش غواصيSCUBA درعمق ۱5-۱0 متري جزيره هنگام (واقع درخليج فارس) ، ايستگاه عرب ها جمع آوري گرديد. گونه هاي مرجان نرم از راسته Alcyonaria و خانواده Ellisellidae و دو گونه .sp Ellisella و Juceella junceaشناسايي شد. عصاره متانولي از دو گونه استخراج گرديد. ﺳﭙﺲ در آزﻣﺎﯾﺸﮕﺎه ﺟﻬﺖ ﺳﻨﺠﺶ ﺧﻮاص ﺳﯿﺘﻮﺗﻮﮐﺴﯿﮏ از آزﻣﻮن ﮐﺸﻨﺪﮔﯽ Artemia salina به روش Brine Shrimp Cytotoxicity Bioassay اﺳﺘﻔﺎده ﮔﺮدﯾﺪ. مرجان نرم دريايي (شلاق دريا) ﻣﺘﺎﺑﻮﻟﯿﺖﻫﺎي ﺛﺎﻧﻮﯾﻪ اي را ﺗﻮﻟﯿﺪ ﻣﯽ ﮐﻨﻨﺪ ﮐﻪ ﺑﺴﺘﻪ ﺑﻪ ﺧﺎﻧﻮاده، ﮔﻮﻧﻪ و ﻣﻨﺎﻃﻖ ﺟﻐﺮاﻓﯿﺎﯾﯽ داراي اﺛﺮات ﮐﺸﻨﺪﮔﯽ ﺑﺮ ﻻرو آرﺗﻤﯿﺎ ﺳﺎﻟﯿﻨﺎ ﻣﯽ ﺑﺎﺷﻨﺪ. همچنين نتايج نشان مي دهد عصاره متانولي دو گونه .sp Ellisella و Juceella junceaﻣﯽ ﺗﻮانند حاوي تركيبات با ارزشي در جهت توليد داروهاي سيتوتوكسيك باشند كه درآزمون هاي آتي مورد استفاده قرار گيرند.

Cytotoxic compounds are factors that directly destroy the cell and affect the various stages of synthesis and activity of nucleic acids and inhibit cell division. The study of cytotoxic effects of methanolic extracts has multiple benefits including less cost, more sensitivity, and less time to do the experiment as well as lower amount of sample required for the experiment. In this study, the corals were collected by SCUBA at a depth of 10-15 m of Hengam Island (Persian Gulf). Two soft coral species, called Ellisella sp. and Juceella juncea from Alcyonaria order and Ellisellidae family were identified. The methanolic extract was extracted from two species.Then in the lab, Artemia salina lethality test was performed to measure cytotoxic properties using Brine Shrimp Cytotoxicity Bioassay. The calculations done by linear regression and probit analysis showed that 50% of Ellisella sp larvae were killed at a concentration of 3.8 mg/ml. In Juceella juncea, a concentration of 3.44 mg/ml resulted in the death of 50% of larvae. Marine soft corals produce secondary metabolites that, depending on family, species and geographical areas have fatal effects on Artemia salina larvae. In addition, our results indicated that the methanolic extract of Ellisella sp. and Juceella juncea could contain valuable compounds to produce cytotoxic drugs to be used in future tests.