Enriched Artemia franciscana nauplii and biomass with soybean lecithin: effects of biochemical and lipid class composition

Enriched Artemia franciscana nauplii and biomass with soybean lecithin: effects of biochemical and lipid class composition

Lecithin is a rich source of phospholipid and phospholipids are considered to increase the growth efficiency, reproduction and evolution of fish and crustaceans. Due to Insufficient synthesis of phospholipids in the aquatic this research was performed to investigate the effect of enriched Artemia franciscana nauplii and biomass with soybean lecithin on proximate and lipid class composition. In the first stage, 200000 nauplii per liter of 26 ppt water were enriched with soya lecithin (74.42% phospholipid) for 24 hours. The enrichment emulsion with the concentration of 0.3 g ml-1 was administered in two doses at T0 and after 12 hours. Adult Artemia (14 days and length 8.50 mm) were transferred to 26 ppt saline water at the density of 3000 individuals per liter. The soybean lecithin emulsion with the concentration of 0.3 g ml-1 was administered to adult Artemia in two rations at T0 and after 3 hours. The enriched Artemia were collected after 6 hours of enrichment and washed thoroughly and stored at -28 for lipid extraction and classes of lipid analysis. The results showed that there was no significant difference in the amount of dry matter and crude protein between Artemia nauplii and biomass enriched with soybean lecithin and un-enriched Artemia (p 0.05). Enrichment with lecithin in both stage, nauplii and biomass, causes a significant increase in the amount of crude lipid (in nauplii from 17.62 to 21.39 and in Artemia biomass from 16.05 to 19.69 %). In terms of lipid classes, phosphatidyl choline, phosphatidyl serine + phosphatidyl inositol were significantly higher in enriched nauplii than unenriched nauplii (p 0.05). In enriched Artemia adult, levels of phosphatidyl ethanolamine and lysophosphatidylcholine were significantly different than control groups. Also enrichment of Artemia nauplii and biomass with soybean lecithin significantly increased total polar lipids (12.96% and 43.72%) and triglycerides (53.35% and 79.9%). In conclusion, according to the results of this study, it can be stated that enrichment with soybean lecithin can improve the content of lipid classes in Artemia nauplii and biomass

Lecithin is a rich source of phospholipid and phospholipids are considered to increase the growth efficiency, reproduction and evolution of fish and crustaceans. Due to Insufficient synthesis of phospholipids in the aquatic this research was performed to investigate the effect of enriched Artemia franciscana nauplii and biomass with soybean lecithin on proximate and lipid class composition. In the first stage, 200000 nauplii per liter of 26 ppt water were enriched with soya lecithin (74.42% phospholipid) for 24 hours. The enrichment emulsion with the concentration of 0.3 g ml-1 was administered in two doses at T0 and after 12 hours. Adult Artemia (14 days and length 8.50 mm) were transferred to 26 ppt saline water at the density of 3000 individuals per liter. The soybean lecithin emulsion with the concentration of 0.3 g ml-1 was administered to adult Artemia in two rations at T0 and after 3 hours. The enriched Artemia were collected after 6 hours of enrichment and washed thoroughly and stored at -28 for lipid extraction and classes of lipid analysis. The results showed that there was no significant difference in the amount of dry matter and crude protein between Artemia nauplii and biomass enriched with soybean lecithin and un-enriched Artemia (p 0.05). Enrichment with lecithin in both stage, nauplii and biomass, causes a significant increase in the amount of crude lipid (in nauplii from 17.62 to 21.39 and in Artemia biomass from 16.05 to 19.69 %). In terms of lipid classes, phosphatidyl choline, phosphatidyl serine + phosphatidyl inositol were significantly higher in enriched nauplii than unenriched nauplii (p 0.05). In enriched Artemia adult, levels of phosphatidyl ethanolamine and lysophosphatidylcholine were significantly different than control groups. Also enrichment of Artemia nauplii and biomass with soybean lecithin significantly increased total polar lipids (12.96% and 43.72%) and triglycerides (53.35% and 79.9%). In conclusion, according to the results of this study, it can be stated that enrichment with soybean lecithin can improve the content of lipid classes in Artemia nauplii and biomass