The effects of using standard medium Guillard (f/2) on biochemical compositions and fatty acid profiles of Haematococcus pluvialis in fresh and brackish water

The effects of using standard medium Guillard (f/2) on biochemical compositions and fatty acid profiles of Haematococcus pluvialis in fresh and brackish water

Using the standard methods for the productions of unicellular algae have high harvest costs and in many cases is not affordable. Therefore chemical fertilizers could be considered as an appropriate alternative for the production of unicellular algae. The green microalgae Haematococcus pluvialis is capable to produce high levels of astaxanthin and fat in resistant cells called spore. The H.pluvialis was obtained from Artemia and Aquaculture Research Institute (AARI), Urmia University, Iran. Three liter flasks were used for cultivating algae. The initial density was set to 4.25 × 104 cells.ml-1. The primary conditions were established for salinity (ppt), pH and temperature, 7.5, 8.00 and 21±1 respectively. Exposure to cold light with light intensity 3000-3500 lux and Guillard s medium (Guillard 1973) for rearing. Each flask was continuously aerated with aeration rate equal to 758 mL per min using filtered pipette. Each treatment was conducted in three replicates as T1 Guillard (in water with salinity of 7.5 ppt) and T2 Guillard (in freshwater). During the cultivation of H.pluvialis in brackish water, the lipid content produced did not exceed more than 35% and so this rearing condition could not be a suitable alternative for lipid production. The protein and carbohydrate contents produced in H.pluvialis cultured by Guillard medium in freshwater and brackish water showed a higher amount, which may probably be due to the spending low energy for osmotic balance in freshwater and also brackish water that has led to higher carbohydrate production. The H.pluvialis fatty acid profiles showed that algae raised in brackish water produced higher ALA than algae cultured in fresh water so that the ALA produced by H.pluvialis in fresh water was less than 0.5% of total FAME. The ARA was also highest in freshwater, so it was not seen in most other rearing conditions. Cultivation of H.pluvialis in freshwater with Guillard (100%) improved the DHA level to more than 11 percent.

Using the standard methods for the productions of unicellular algae have high harvest costs and in many cases is not affordable. Therefore chemical fertilizers could be considered as an appropriate alternative for the production of unicellular algae. The green microalgae Haematococcus pluvialis is capable to produce high levels of astaxanthin and fat in resistant cells called spore. The H.pluvialis was obtained from Artemia and Aquaculture Research Institute (AARI), Urmia University, Iran. Three liter flasks were used for cultivating algae. The initial density was set to 4.25 × 104 cells.ml-1. The primary conditions were established for salinity (ppt), pH and temperature, 7.5, 8.00 and 21±1 respectively. Exposure to cold light with light intensity 3000-3500 lux and Guillard s medium (Guillard 1973) for rearing. Each flask was continuously aerated with aeration rate equal to 758 mL per min using filtered pipette. Each treatment was conducted in three replicates as T1 Guillard (in water with salinity of 7.5 ppt) and T2 Guillard (in freshwater). During the cultivation of H.pluvialis in brackish water, the lipid content produced did not exceed more than 35% and so this rearing condition could not be a suitable alternative for lipid production. The protein and carbohydrate contents produced in H.pluvialis cultured by Guillard medium in freshwater and brackish water showed a higher amount, which may probably be due to the spending low energy for osmotic balance in freshwater and also brackish water that has led to higher carbohydrate production. The H.pluvialis fatty acid profiles showed that algae raised in brackish water produced higher ALA than algae cultured in fresh water so that the ALA produced by H.pluvialis in fresh water was less than 0.5% of total FAME. The ARA was also highest in freshwater, so it was not seen in most other rearing conditions. Cultivation of H.pluvialis in freshwater with Guillard (100%) improved the DHA level to more than 11 percent.