پديدآورندگان :
Abedia Sepideh Faculty of New Sciences and Technologi es, University of Tehran, Tehran, Iran , Razi Astaraiea Fatemeh Faculty of New Sciences and Technologi es, University of Tehran, Tehran, Iran , Ghobadianb Barat Department of Biosystems Engineering, University of Tarbiat Modares, Tehran, Iran , Tavakolic Omid School of Chemical Engineering, College of Engineering, University of Tehran, Tehran, Iran , Jalilia Hassan Faculty of New Sciences and Technologi es, University of Tehran, Tehran, Iran , Greenwelld H. C hris d Department of Earth Sciences, Durham University, Durham, DH1 3LE, UK. , Chivasa Stephen e Department of Biosciences, Durham University,South Road, Durham, DH1 3LE, UK
كليدواژه :
Trichorm us variabilis , Nitrogen Fixation , N , SUFFICIENT (BG11) , N , DEFICIENT (BG0)
چكيده فارسي :
HYDROGEN IS CONSIDERED AS A FUTURE FUEL DUE TO ENVIRONMENTAL ADVA NTAGES COMPARED TO CONVENTIONAL FO SSIL FLUES ESPECIALLY WHEN IT IS GENERA TED BY BIOLOGICAL SYSTEMS. TRICHORMUS VARIABILIS (SYN. ANABAENA VARIABILIS) BLU E-GREEN MICROALGAE CONSIDERED AS AN EFFICIENT MICROORGANISM GROWN IN BOTH CON DITIONS OF HIGH AMMONIUM WASTEWATER STREAMS AND NITROGEN DEPLETION MEDIUM OWING HIGH CAPABILITY OF NITROGEN FIX ATION POTENTIAL. HYDROGEN IS GENERATED A S A BY-PRODUCT DURING NITROGEN FIXA TION METABOLISM WHEN IT IS SHIFTED FROM N-SUFFICIENT (BG11) TO N-DEFICIENT (BG0) MEDIUM. THEREFORE AMMONIUM IS A POWERFUL REGULATOR IN NITROGEN FIXATIO N AND HYDROGEN GENERATION MECHANISM OF THIS SPECIES. WITH A GENOME CONTAINING ~5724 OPEN READING FRAMES, T. VA RIABILIS REPRESENTS A VERY SIMPLE ORGANISM IN WHICH SIGNALS CONTROLLING NI TROGEN FIXATION COULD BE STUDIED. HERE OF, A PROTEIN EXPRESSION THROUGH ACTIVATION OF NITROGEN FIXATION GENES IS INVESTIGATED VIA PROTEOMIX TO (I) CHARACTERIZA TION OF PROTEOME RESPONSE TO N-DEP LETION MEDIUM AND (II) IDENTIFY OTH ER KEY METABOLISMS AFFECTED BY NITROG EN FIXATION WHICH INDUCE HYDROGEN GENERATION. RESULTS SHOWED AMONG 184 IDE NTIFIED PROTEINS, 20 PROTEINS WERE SIGNIFICANTLY (P 0.005) CHANGED. IT WAS DE MONSTRATED THAT IN ADDITION TO NITRO GENAZE ENZYMES, EXPRESSION OF SOME PHOTOSYNTHESIS, ENERGY, CARBOHYDRATE, GENE TIC AND ENVIRONMENTAL PROCESSING GENE S HAVE CHANGED THROUGH SHIFTING FROM BG11 TO BG0. FURTHER RESEARCHES HAVE T O BE UNDER TAKEN NECESSARILY IN THE FU TURE TO IDENTIFY ALL PROTEINS SIGNIFICA NTLY ALTERED DURING HYDROGEN GENERATION VIA NITROGEN FIXATION MECHANISM.
چكيده لاتين :
HYDROGEN IS CONSIDERED AS A FUTURE FUEL DUE TO ENVIRONMENTAL ADVA NTAGES COMPARED TO CONVENTIONAL FO SSIL FLUES ESPECIALLY WHEN IT IS GENERA TED BY BIOLOGICAL SYSTEMS. TRICHORMUS VARIABILIS (SYN. ANABAENA VARIABILIS) BLU E-GREEN MICROALGAE CONSIDERED AS AN EFFICIENT MICROORGANISM GROWN IN BOTH CON DITIONS OF HIGH AMMONIUM WASTEWATER STREAMS AND NITROGEN DEPLETION MEDIUM OWING HIGH CAPABILITY OF NITROGEN FIX ATION POTENTIAL. HYDROGEN IS GENERATED A S A BY-PRODUCT DURING NITROGEN FIXA TION METABOLISM WHEN IT IS SHIFTED FROM N-SUFFICIENT (BG11) TO N-DEFICIENT (BG0) MEDIUM. THEREFORE AMMONIUM IS A POWERFUL REGULATOR IN NITROGEN FIXATIO N AND HYDROGEN GENERATION MECHANISM OF THIS SPECIES. WITH A GENOME CONTAINING ~5724 OPEN READING FRAMES, T. VA RIABILIS REPRESENTS A VERY SIMPLE ORGANISM IN WHICH SIGNALS CONTROLLING NI TROGEN FIXATION COULD BE STUDIED. HERE OF, A PROTEIN EXPRESSION THROUGH ACTIVATION OF NITROGEN FIXATION GENES IS INVESTIGATED VIA PROTEOMIX TO (I) CHARACTERIZA TION OF PROTEOME RESPONSE TO N-DEP LETION MEDIUM AND (II) IDENTIFY OTH ER KEY METABOLISMS AFFECTED BY NITROG EN FIXATION WHICH INDUCE HYDROGEN GENERATION. RESULTS SHOWED AMONG 184 IDE NTIFIED PROTEINS, 20 PROTEINS WERE SIGNIFICANTLY (P 0.005) CHANGED. IT WAS DE MONSTRATED THAT IN ADDITION TO NITRO GENAZE ENZYMES, EXPRESSION OF SOME PHOTOSYNTHESIS, ENERGY, CARBOHYDRATE, GENE TIC AND ENVIRONMENTAL PROCESSING GENE S HAVE CHANGED THROUGH SHIFTING FROM BG11 TO BG0. FURTHER RESEARCHES HAVE T O BE UNDER TAKEN NECESSARILY IN THE FU TURE TO IDENTIFY ALL PROTEINS SIGNIFICA NTLY ALTERED DURING HYDROGEN GENERATION VIA NITROGEN FIXATION MECHANISM.
