Transcript analysis of some key genes involved in oil biosynthesis in Chalmydomonas

Edible oils are of particular national interest according to the vast market use. Next to oil seed crops, alternative sources of edible oils are always can be beneficial to the industry. Micr oalgae have the potential of producing varieti es of fatty acids and are amenable to improvements via genetic engineering. The produced oils can be used in varieties of industries and therefore such organisms promise greater production using fewer sources of oil and arable lands. Oil accumulation in microalgae is being i nduced mostly through environmental stresses inc luding mineral insufficiency such as sulfur, nitrogen, and phosphorus; producing up to 80% (w/w of biomass) in some species. Many studie s have been carried out to identify the key genes involved in oil biosynthesis in microalgae (1-9). Here, following and through bioinformatics analysis, key genes, LPAAT, KASII and KASIII, were selected that here was no prior knowledge on their fluctuations during oil accumulation o n Chalmydomonas. Real-Time PCR was used to do a comparative analysis between normal and nitrogen starved conditions and the data were normalized against rack1.The aim of th is study was to better identify the key genes in oil biosynthesis pathway in chlamydomon as. In this study results showed that LPAAT and KAS II were upregulated and KASIII was dow nregulated

Edible oils are of particular national interest according to the vast market use. Next to oil seed crops, alternative sources of edible oils are always can be beneficial to the industry. Micr oalgae have the potential of producing varieti es of fatty acids and are amenable to improvements via genetic engineering. The produced oils can be used in varieties of industries and therefore such organisms promise greater production using fewer sources of oil and arable lands. Oil accumulation in microalgae is being i nduced mostly through environmental stresses inc luding mineral insufficiency such as sulfur, nitrogen, and phosphorus; producing up to 80% (w/w of biomass) in some species. Many studie s have been carried out to identify the key genes involved in oil biosynthesis in microalgae (1-9). Here, following and through bioinformatics analysis, key genes, LPAAT, KASII and KASIII, were selected that here was no prior knowledge on their fluctuations during oil accumulation o n Chalmydomonas. Real-Time PCR was used to do a comparative analysis between normal and nitrogen starved conditions and the data were normalized against rack1.The aim of th is study was to better identify the key genes in oil biosynthesis pathway in chlamydomon as. In this study results showed that LPAAT and KAS II were upregulated and KASIII was dow nregulated