Carbohydrate preference and effect of cultivation parameters on the growth and fatty acid profile of a newly isolated microorganism Aurantiochytrium sp. strain SHY

There are limited studies available on the effect of different culture parameters on growth and the fatty acid profile of Aurantiochytrium. In this research, an investigation has been conducted on the effect of different carbon sources, nitrogen sources, and temperature on the dry cell weight and composition of fatty acids in a newly isolated strain of Aurantiochytrium . Glucose produced more biomass, but galactose was more suitable for lipid formation. Galactose promoted the highest production of fatty acids (36.4%), but the ratio of docosahexaenoic acid to fatty acids was 19.05% that is less than glycerol. With an increased glucose concentration, EPA is considerably higher and DHA is only slightly increased. This is the first time a study has been carried out on this new isolate wherein a mixed carbon source was used in the culture media and the results showed the microorganism’s preferences for carbohydrate consumption is in the following pattern: glucose galactose fructose sucrose. The meat extract was the most suitable nitrogen source for biomass development(7g/L), but the maximum docosahexaenoic acid to fatty acids ratio(25%) was achieved when the microorganism consumed monosodium glutamate as a nitrogen source. The strain produced more biomass at 25°C, and lowering the temperature achieved a higher docosahexaenoic acid formation.The results of this study show that the strain of Aurantiochytrium sp. shy is a potential candidate for food industries and in aquaculture.

There are limited studies available on the effect of different culture parameters on growth and the fatty acid profile of Aurantiochytrium. In this research, an investigation has been conducted on the effect of different carbon sources, nitrogen sources, and temperature on the dry cell weight and composition of fatty acids in a newly isolated strain of Aurantiochytrium . Glucose produced more biomass, but galactose was more suitable for lipid formation. Galactose promoted the highest production of fatty acids (36.4%), but the ratio of docosahexaenoic acid to fatty acids was 19.05% that is less than glycerol. With an increased glucose concentration, EPA is considerably higher and DHA is only slightly increased. This is the first time a study has been carried out on this new isolate wherein a mixed carbon source was used in the culture media and the results showed the microorganism’s preferences for carbohydrate consumption is in the following pattern: glucose galactose fructose sucrose. The meat extract was the most suitable nitrogen source for biomass development(7g/L), but the maximum docosahexaenoic acid to fatty acids ratio(25%) was achieved when the microorganism consumed monosodium glutamate as a nitrogen source. The strain produced more biomass at 25°C, and lowering the temperature achieved a higher docosahexaenoic acid formation.The results of this study show that the strain of Aurantiochytrium sp. shy is a potential candidate for food industries and in aquaculture.