Multi-spectroscopy of the interaction behavior between calf thymus DNA and Rebeccamycin in binary and ternary approaches

DNA binding studies have remarkable relevance and implications in drug design aspect and cancer chemotherapy. In eukarute cells, histones play important roles of the biological function of DNA. The interaction between Rebeccamycin and calf thymus DNA (ct DNA), in the absence and presence of H1 was investigated by using multi-spectroscopic techniques. The fluorescence and circular dichroism spectroscopic indicated the Rebeccamycin interacted with ct DNA in the absence of H1 in an intercalator binding mode. In the presence of H1, ct DNA-Rebeccamycin complex revealed intercalator and groove binding modes with dominant of intercalator role.