پديدآورندگان :
Shakeria S samaneh.shakeri9296@gmail.com University of Guilan, , Rasht, Iran , Dezhampanah H h.dpanah@guilan.ac.ir University of Guilan, , Rasht, Iran , Firouzia R h.dpanah@guilan.ac.ir University of Guilan, , Rasht, Iran
كليدواژه :
BSA , Fluorescence , FTIR , interaction
چكيده فارسي :
: The interaction of disperse azo dye with bovine serum albumin (BSA) , in 20 mM phosphate buffer pH 7.4 was investigated using advanced techniques including fluorescence, Fourier transform infrared (FT-IR). The fluorescence quenching measurements showed a single binding site on BSA for dispers azo dye with the binding constant (Kb) value equals to 3/39×104 at 25°C. The results showed that quenching mechanism of BSA by dispers azo dye was of dynamic type.
