پديدآورندگان :
Benvidi Ali abenvidi@yazd.ac.ir Department of Chemistry, Yazd University, Yazd, Iran, , Asoodeh Amin Department of Chemistry, Yazd University, Yazd, Iran , Dadras Abbas Department of Chemistry, Yazd University, Yazd, Iran , Rezaeinasab Masoud Department of Chemistry, Yazd University, Yazd, Iran , Abbasi Saleheh Department of Chemistry, Yazd University, Yazd, Iran , Dehghan Tezerjani Marzieh Department of Chemistry, Yazd University, Yazd, Iran , Sedighipoor Maryam Department of Chemistry, Isfahan University of Technology, Isfahan, I. R. Iran
چكيده فارسي :
Penicillamine as a pharmaceutical of the chelator class is mainly used for the treatment of rheumatoid arthritis and mercury poisoning. [1] Also, it is classified as a chelating agent which is significant in treating some diseases such as Wilson’s disease. [2] Therefore, the trace determination of this compound is very important. Adrenaline is a catecholamine which is important in the functioning of the central nervous system as well as renal, hormonal, and cardiovascular systems. [3] Due to this fact that there are many life phenomena which are related to the concentration of Adrenaline in the blood, the quantitative determination of Adrenaline in different human fluids is a significant method for making diagnoses and controlling medicines. [4] In the present work, we prepared a carbon paste modified electrode with Bisbenzoylacetone-ethylenediimine cobalt (II) (CO (II) - BBE) and reduced graphene oxide (Co (II)/RGO/CPE) as an electrochemical sensor for electrochemically sensitive and selective detection of penicillamine in the presence of adrenaline. The results showed that Co (II)/RGO/CPE had high electrocatalytic activity for the electrooxidation of penicillamine in aqueous buffer solution (pH = 7.0). The electrocatalytic oxidation peak currents increase linearly with Penicillamine concentrations over the concentration ranges of 0.5-104.5 µM using square wave voltammetric method. Under the optimum conditions, the detection limit for Penicillamine was obtained to be 0.168 µM. The modified electrode was used for the determination of Penicillamine and adrenaline simultaneously in real sample. Since two materials have close oxidation peaks, the obtained square wave voltammetric (SWV) signals indicated overlap at the modified electrode. So, to solve this problem a suitable chemometric method of multivariate curve resolution based on the alternative least squares (MCR-ALS) was used. As known, MCR-ALS is useful tool for resolution of different species present in the equilibrium systems. It let us to extract the concentration profile and pure spectra in a mixture from a set of spectra with various composition. [5] Using this chemometric method led to solving the overlapping SWV signals of penicillamine and adrenaline and consequently simultaneous determination of their binary mixtures.
