پديدآورندگان :
Tavakkoli Hamed Professor Massoumi Laboratory, Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran , Akhond Morteza Professor Massoumi Laboratory, Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran , Ghorbankhani Gholam Abbas Division of Biotechnology, Department of Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz Iran , Absalan Ghodratollah gubsulun@yahoo.com Professor Massoumi Laboratory, Department of Chemistry, College of Sciences, Shiraz University, Shiraz 71454, Iran;
كليدواژه :
Electrochemical biosensor , Platinum nanoflowers , Zein nanoparticles , Multiwall carbon nanotubes , living tumor cells
چكيده فارسي :
During the past two years, due to progress in nanotechnology, a great concern has arisen for monitoring of living tumor cells using hydrogen peroxide (H2O2) as a reliable biomarker by employing different electrode devices with various nanocomposite modifiers [1]. In this study, an efficient free-enzyme biosensor was constructed using electrochemically deposition of platinum nanoflower (PtNF) [2] on a glassy carbon electrode (GCE) modified with multiwall carbon nanotubes/zein bio-nanocomposite which was characterized by X-ray diffraction, field emission electron microscopy as well as transmission electron microscopy. The electrochemical studies and measurements were carried out by using cyclic voltammetry, amperometry and linear sweep voltammetry. Under an amperometric sensing, the sensor provided attractive electrocatalytic performances for sensitive and selective enzyme-free detection of H2O2 within a linear concentration range of 29.0 nM to 215.5 μM H2O2 with a detection limit of 15.0 nM H2O2 in 0.1 M phosphate buffer at pH 7.2. Furthermore, the biosensor was successfully checked for being utilized in living tumor cells monitoring through nanomolar level probing of H2O2 as released from both human dermal fibroblasts (HDF) and human hepatocellular carcinoma cells (HepG2; liver cancer cell).
