پديدآورندگان :
Rafipour Ronak r.rafipour@iauksh.ac.ir Department of Chemistry, Kermanshah Branch, Islamic Azad University, Kermanshah, Iran; , Kashanian Soheila Faculty of Chemistry, SensorandiosensorResearch Center (SBRC) Nanoscience and Nanotechnology Research Center (NNRC), Razi University, Kermanshah, Iran
چكيده فارسي :
Ferritin is a globular protein complex consisting of 24 protein subunits to form cage architecture of 12 nm diameter with an interior cavity of 8 nm diameter in which a hydrated ferric oxide/phosphate is mineralized. Mammalian ferritins consist of H- and L-chains (heavy and light chains, respectively). The H-subunit encodes the ferroxidase site, which oxidizes the iron (II) ion and produces the iron (III) hydroxide cores. In the assembled form, ferritin L chains are known to have a greater negative charge on their interior, with clusters of glutamates and aspartates that make up the nucleation site. ferritin has been used as a biological template for nanomaterial synthesis. In addition to Fe, ferritin can also encapsulate other electroactive materials such as CdS, Co, Co:Pt, Ni, Pd, Mn, and Pt, making ferritin suitable for electrochemical biosensor fabrication[1-2]. In this study, manganese nanoparticles (MnNPs) were created within horse spleen apoferritin (HsAFr) cavity nanotemplates. Then, these MnNP-HsAFr were incorporated into the surface of a carbon electrode using a two-step process. CS polymer film containing MCWNTs was deposited on the electrode surface by drop coating. Then, MnNPs–HsAFr was immobilized onto MWCNTs entrapped into CS matrices by covalent glutaraldehyde (GA) cross-linking. GA has had great success because it is commercially available and inexpensive in addition to its high reactivity. It reacts rapidly with amine groups at around neutral pH and is more efficient than other aldehydes in generating thermally and chemically stable cross-links. [3]. CS contains large numbers of –NH2 and –OH, and it has been used as a modifying reagent to construct modified electrode due to its excellent biocompatibility, nontoxicity, cost effectiveness, and high mechanical strength. CS has been selected as an orientation directing matrix due to possessing large quantities of amino and hydroxyl groups on its units, which have a strong binding ability to biomolecules. However, it is a nonconducting biomaterial, therefore compounds with good conductivity such as CNTs need to be combined with CS to achieve a high-performance biosensor [4]. Generally speaking, two immobilization methods were postulated.
