Redox Reaction of Silver Nanoparticles Coated Graphite as an Electrochemical Signature for Determination of Thiopental Sodium

Thiopental is an ultra-short-acting barbiturate used to induce anesthesia in man and animals. .It is used for intensive-care patients with head injuries to reduce intracranial hypertension and is useful to prevent and treat brain ischemia [1]. Different methods for the determination of thiopentontal sodium have been reported. However, most procedures suffer from the lack in some simplicity, sensitivity, and selectivity. Therefore,   it is necessary to developing new methods for determination of thiopentontal with good sensitivity and selectivity. Among all noble metal nanoparticle, silver nanoparticles (AgNPs) keep on to be of enormous current research attention because of their catalytic properties [3]. AgNP traditionally been explored to employ as catalysts in various reactions and also Ag exhibits the highest electrical and thermal conductivity among all the metals[4]. Based on our knowledge, use of electrochemical sensors for Thiopental determination was rare. In this work, we designed a new electrochemical sensor for the determination of TPS. Some important factors such as pH, ratio of AgNPs-graphite in the electrode, pretreatment potential, and accumulation time were optimized. Under optimized experimental conditions, relationship between current reduction of the electrode and concentration of TPS was linear in range of 10-10 to 5×10-6 molL-1 .To investigate repeatability of the method it was tested nine time under the same condition and RSD = 1.24 were obtained for this sensor. In summary, we have developed a novel electrochemical sensor based on synthesis of AgNPs coated graphite for determination of TPS. The method is simple, time saving, and very sensitive over the reported data. It can be used successfully to assay the drug in dosage form and in biological samples at trace levels.