Voltammetric Sensor for Determination of Morin at Stacked Cu Nanoparticles Immobilized on l- Arginine Film Nanocomposite Modified GCE

Metallic nanoparticles (MNPs) have also drawn particular attention due to their high surface area, effective mass transportation, catalytic properties and control over local microenvironment [1, 2]. Conductive polymers have attracted attention as a proper choice for the modification of electrodes’ surfaces. Among different methods for preparing polymer-modified electrodes, electropolymerization is a good choice to immobilize the polymer, as the film thickness and its permeation characteristics can be controlled. Formation of metal nanoparticles (MNPs) in a polymer matrix is a popular tool for design of metal nanoparticles and polymers frameworks (MNPPFs). Properties of polymer can be greatly altered by incorporating MNPs [3].To obtain MNPPFs with well-defined and reproducible properties, one should achieve suitable control over MNPs growth, particle size distribution, and particle–interface interactions. In this work, a sensitive and convenient electrochemical sensor based on stacked arginine film and copper nanoparticles composite modified glassy carbon electrode was developed for the determination of morin. Electrochemical investigation of the modified electrode are achieved using cyclic voltammetry, differential pulse voltammograms. The effect of several experimental variables, such as pH of the supporting electrolyte and number of electropolymerization cycles were optimized by monitoring the CV response of the modified electrode toward morin. Under the optimized conditions, a significant electrochemical improvement was observed toward the electrooxidation of morin on the modified electrode surface relative to the unmodified electrode. The oxidation peak current of morin was proportional to its concentration over the range from 0.2 to 9.8 µM. The limit of detection was evaluated to be 0.16 µM. The developed nanosensor was applied to the samples of juice for determination of morin with high sensitivity and high selectivity.