Rapid, Sensitive and trace analysis of Ceftriaxone using highly fluorescent carbon dots synthesized by hydrothermal method from Chicken meat, and modification of this nanoprobe with silver nanoparticles as a novel highly Sensitive “Turn-On” Fluorescent probe for morphine Sensing

Carbon dots (CDs) have attracted considerable attention recently because of their good stability, low cytotoxicity, high biocompatibility and flexibility in surface functionalization compared to those of organic fluorophores and semiconductor quantum dots [1-3]. Ceftriaxone (CFX) is a semi-synthetic antibacterial antibiotic that is a derivative of the third generation of cephalosporins. High-performance liquid chromatography is the method most commonly used for the determination of CFX. In this work a simple, rapid, selective and sensitive method was proposed for determining of ceftriaxone. Morphine (MRF) is a powerful narcotic analgesic and highly addictive .It is used primarily to treat both acute and chronic severe pain. It is also used for pain due to myocardial infarction and for labor pains [4].In this report, a novel fluorescent sensing Carbon dots (CDs) was synthesised by a simple, green, and low-cost method using a one-pot hydrothermal treatment of chicken meat. The as-prepared carbon dot has exceptional advantages including high fluorescent quantum yield and satisfactory chemical stability. The synthesized CDs were characterized using various techniques as transmission electron microscopy (TEM), UV-Visible spectroscopy and photoluminescence spectroscopy. More strikingly, as-synthesized CDs generate strong response to ceftriaxone and gives rise to the fluorescence quenching. This phenomenon was used to develop a fluorescent method for facile detection of ceftriaxone with a linear range from 0.9 to 140 nM and a detection limit of 4.4×10-10M, and further extended to measure urine samples with satisfactory recoveries. Eventually, carbon dots-modified silver nanoparticles (CDs-Ag NPs) were successfully prepared for rapid and selective determination of morphine.The CDs-AgNPs fluorescent probe illustrates a linear detection range towards morphine from 0.04 μg/ mL to 0.40 μg/ mL with the detection limit of 0.003μg/ mL. The results showed that this probe is facile, rapid and offers high sensitivity, selectivity, repeatability and sustainability.