شماره ركورد كنفرانس :
4887
عنوان مقاله :
Recombinant Hepatitis B surface Antigen separation from Clarified debris-free lysate using Aerosil
پديدآورندگان :
Hosseini Seyyed Nezamedin Production Research complex of Pasteur Institute of IRAN , Gheysari Parisa Chemical Eng.Departmant, Razi University, Kermanshah, IRAN , Khatami Maryam Production Research complex of Pasteur Institute of IRAN , Rezaei Davood Chemical Eng.Departmant, Guilan University, Rasht, IRAN
كليدواژه :
Adsorption capacity , Areosil , Optimaization
عنوان كنفرانس :
پانزدهمين كنگره ملي مهندسي شيمي ايران
چكيده فارسي :
Recombinant Hepatitis B surface Antigen (r-HBsAg) adsorption/desorption capacity on nano silica colloidal (Aerosil 380) adsorbent was investigated using batch process. Effects of five parameters including Adsorption pH, desorption pH, adsorption time, agitation speed and initialantigen concentration were studied. Optimal conditions were 5 and 9 for adsorption pH and desorption pH respectively, 4hr for adsorption time, 600 rounds per minutes (rpm) of agitation speed and 40μg/ml initial antigen concentration. In these conditions r-HBsAg adsorbtion/desorption efficiency on Aerosil was obtained 91%. The results also indicated that Langmuir isotherm provided the best correlation of experimental data for adsorbent
