پديدآورندگان :
Rezaei Javad Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran , RajabiBazl Masoumeh Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran , Mousavi Seyedlatif Department of Biology, Faculty of Basic Science, Shahed University, Tehran, Iran , Rafiei Dehbidi Gholamreza Department of Clinical Biochemistry, Faculty of Medicine, Shahid Beheshti University of Medical Science, Tehran, Iran
كليدواژه :
prostate cancer , PSMA , scFv , phage display
چكيده فارسي :
The researches in the field of radioimunotherapy and chemoimunotherapy are based on monoclonal antibody production and demonstrate that these methods are effective in inhibiting the growth of prostate tumor. Today hybridoma and phage display methods are used for production of monoclonal antibodies. Phage display method is widely used for monoclonal antibody production because of its inexpensivity. One of the methods used in isolation of monoclonal antibodies is use of artificial antibody libraries that include more than 100 million different scFv fragments coloned in ampicillin resistance phagmid vector and have transformed into E. coli cell ( scFv fragments contain single polypeptide with VL and VH domains that have been connected to each other’s with flexible connector (serine-glycine)) scFv fragments have various merits, for example they have small size, high perfusion, high yield production and non-immunogenicity (because of Fc fragment elimination), thus they can be used for therapeutic purposes .
