شماره ركورد كنفرانس :
4522
عنوان مقاله :
Molecular typing of Leishmania major parasite in the main vector of leishmaniasis in Khuzestan by targeting the ITS-rDNA gene
پديدآورندگان :
Taslimian Roozbeh Molecular Systematics Laboratory, Deparment of Parasitology, Pasteur Institute of Iran, Tehran. , Parvizi Parviz Molecular Systematics Laboratory, Deparment of Parasitology, Pasteur Institute of Iran, Tehran. , Reza Esmaili Dr. Ahmad Molecular Systematics Laboratory, Deparment of Parasitology, Pasteur Institute of Iran, Tehran. , Spotin Adel Molecular Systematics Laboratory, Deparment of Parasitology, Pasteur Institute of Iran, Tehran
كليدواژه :
Leishmania major , Sandfly , Khuzestan , ITS , rDNA , PCR , RFLP.
عنوان كنفرانس :
هشتمين همايش بيوتكنولوژي جمهوري اسلامي ايران و چهارمين همايش ملي امنيت زيستي
چكيده فارسي :
Background amp; Objective:Leishmania major is an underlying agent of zoonotic cutaneous leishmaniasis in an endemic focus of Khuzestan. In this study, we diagnose Leishmania parasites by targeting of ITS-rDNA gene because there is no molecular typing and characterization which has been carried out on P. papatasi as a main vector of L. major yetMaterials amp; Methods:Sandflies were caught using sticky traps and light traps. After dissecting, sandflies wereidentified. DNA was extracted and ITS-rDNA gene of Leishmania in sandflies amplified byPCR methods and by RFLP and sequencing then using molecular software Leishmania majorwas definitively approved.Results:Among 1500 sandflies, 198 female P. papatasi were selected as gravid, semi gravid and freshfed. DNA was extracted from all of them and the ITS-rDNA gene was amplified. About 8percent of sandflies was infected with Leishmania parasites. After RFLP and sequencing samples Leishmania major was identified in all cases.Conclusions:The sequences results obtained in this study compared with similar cases recorded in the GenBank and confirmed.
