شماره ركورد كنفرانس :
4522
عنوان مقاله :
Molecular typing of sandflies, proven and possible vectors of leishmaniasis in Khuzestan province, using two genes elongation factor_1α and cytochrome b
پديدآورندگان :
Ebrahimi Sahar Molecular Systematics Laboratory, Parasitology Department ,Pasteur Institute of Iran.Islamic Azad University of Tehran, Medical Science Branch. , Parvizi Parviz Molecular Systematics Laboratory, Parasitology Department ,Pasteur Institute of Iran. , Belgheiszadeh Hamid Islamic Azad University of Tehran, Medical Science Branch. , Amraee Kamyar Department of Medical Entomology and Vector Control. Ahvaz Jundishapour University of Medical Sciences, Ahvaz, Iran
كليدواژه :
Molecular typing sandflies , Cytochrome b , Elongation factor_1α , Khuzestan.
عنوان كنفرانس :
هشتمين همايش بيوتكنولوژي جمهوري اسلامي ايران و چهارمين همايش ملي امنيت زيستي
چكيده فارسي :
Summary and Objective:Sandflies are vectors of leishmaniasis in human in rural areas, Khuzestan. Some of Phlebotominae sandflies with morphological characters are unable to distinguish but withmolecular methods are applicable.Materials and Methods:In adult sandflies activity seasons, samples were collected using sticky trap, aspirator and CDC light trap from different locations of Khuzestan. After dissection, sandflies were mounted. DNA was extracted. Two genes of elongation factor_1α and Cytochrome b genes were amplified, typed and analyzed using molecular software.Results:About 3000 sandflies were collected, first identified based on morphological keys. 150 different sandfly species were selected, DNA was extracted and two genes amplified by PCR and sequenced the fragments. These samples were contained main and possibility vectors of leishmaniasis in KhozestanDiscussion:Using morphological characters in some female sandfly species are indistinguishable. By employing elongation factor_1α and Cytochrome b genes not only different sandfly species were identified but also different haplotypes of each sandfly species were found.
