ﺑﺮرﺳﯽ اﺛﺮ ﻧﺎﻧﻮ ذره دي اﮐﺴﯿﺪ ﺗﯿﺘﺎﻧﯿﻮم ﺑﺮ ﺑﺮﺧﯽ از ﺧﺼﻮﺻﯿﺎت آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﮔﯿﺎه داروﯾﯽ ﺑﻪ ﻟﯿﻤﻮ .Aloysia citriodora L در ﺳﻄﻮح ﻣﺨﺘﻠﻒ ﺷﻮري

Assessment of nano particle titanium dioxide on some antioxidant properties of Aloysius citriodora L. under salt stress

در اﯾﻦ ﭘﮋوﻫﺶ ﺑﻪ ﻣﻨﻈﻮر ﺑﺮرﺳﯽ اﺛﺮ ﻧﺎﻧﻮ ذرات دي اﮐﺴﯿﺪ ﺗﯿﺘﺎﻧﯿﻮم ﺑﺮ ﺑﺮﺧﯽ از ﺧﺼﻮﺻﯿﺎت آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﮔﯿﺎه داروﯾﯽ ﺑﻪ ﻟﯿﻤﻮ در ﺳﻄﻮح ﻣﺨﺘﻠﻒ ﺷﻮري آزﻣﺎﯾﺶ ﻓﺎﮐﺘﻮرﯾﻞ در ﻗﺎﻟﺐ ﻃﺮح ﮐﺎﻣﻼً ﺗﺼﺎدﻓﯽ ﺑﺎ ﺳﻪ ﺗﮑﺮار اﺟﺮا ﮔﺮدﯾﺪ. ﻓﺎﮐﺘﻮر اول ﺷﺎﻣﻞ ﻧﺎﻧﻮ ذره دي اﮐﺴﯿﺪﺗﯿﺘﺎﻧﯿﻮم در ﭘﻨﺞ ﺳﻄﺢ )ﺻﻔﺮ، 50، 100، 200 و 400 ﭘﯽﭘﯽام( و ﻓﺎﮐﺘﻮر دوم ﺷﺎﻣﻞ ﺷﻮري در ﺳﻪ ﺳﻄﺢ )ﺻﻔﺮ، 75 و 150 ﻣﯿﻠﯽﻣﻮﻻر( اﻋﻤﺎل ﮔﺮدﯾﺪ. ﻧﺘﺎﯾﺞ ﺗﺠﺰﯾﻪ وارﯾﺎﻧﺲ ﺑﯿﺎﻧﮕﺮ آن ﺑﻮد ﮐﻪ ﺗﻨﺶ ﺷﻮري ﺳﺒﺐ ﮐﺎﻫﺶ ﺻﻔﺎت آﻧﺘﯽ اﮐﺴﯿﺪاﻧﯽ ﺷﺪ اﻣﺎ اﺛﺮ ﺳﺎده ﻧﺎﻧﻮ ذره دي اﮐﺴﯿﺪ ﺗﯿﺘﺎﻧﯿﻮم روﻧﺪ اﻓﺰاﯾﺸﯽ را ﻧﺸﺎن داد. ﻣﻘﺎﯾﺴﻪ ﻣﯿﺎﻧﮕﯿﻦ ﺻﻔﺎت ﻣﻘﺪار ﮐﻠﺮوﻓﯿﻞ b ،a و ﮐﻞ، ﮐﺎروﺗﻨﻮﺋﯿﺪ و آﻧﺘﻮﺳﺎﻧﯿﻦ ﺣﺎﮐﯽ از آن ﺑﻮد ﮐﻪ ﺑﺎ اﻓﺰاﯾﺶ ﻏﻠﻈﺖ ﻧﺎﻧﻮ ذره دي اﮐﺴﯿﺪ ﺗﯿﺘﺎﻧﯿﻮم ﺻﻔﺎت ﻣﻮرد ﻧﻈﺮ روﻧﺪ اﻓﺰاﯾﺸﯽ ﻣﻌﻨﯽ داري را ﻧﺸﺎن داد اﻣﺎ ﺑﺎ اﻓﺰاﯾﺶ ﺳﻄﻮح ﺷﻮري ﻣﺤﺘﻮاي ﮐﻠﺮوﻓﯿﻞ ﮐﺎﻫﺶ ﯾﺎﻓﺖ. ﺑﺮ اﺳﺎس ﯾﺎﻓﺘﻪ ﻫﺎي ﭘﮋوﻫﺶ ﺣﺎﺿﺮ ﻣﯽﺗﻮان ﭘﯿﺸﻨﻬﺎد ﮐﺮد ﺗﺎ از ﻧﺎﻧﻮذره دي اﮐﺴﯿﺪ ﺗﯿﺘﺎﻧﯿﻮم در ﺟﻬﺖ ﺑﻬﺒﻮد ﺻﻔﺎت ﻣﺨﺘﻠﻒ ﮔﯿﺎﻫﯽ ﻧﻈﯿﺮ ﻣﻮرﻓﻮﻟﻮژي، ﻓﯿﺰﯾﻮﻟﻮژي و ﺑﯿﻮﺷﯿﻤﯿﺎﯾﯽ در ﺷﺮاﯾﻂ ﺗﻨﺶﻫﺎي ﻏﯿﺮ زﯾﺴﺘﯽ اﺳﺘﻔﺎده ﮔﺮدد.

In this study, the assessment of titanium effect on some antioxidant properties of Aloysia citriodora L. was performed based on completely factorial randomized design experiment with three replications. The first and second factors were titanium (five levels of zero, 50, 100, 200 and 400 ppm) and salt stress (three levels of zero, 75 and 150 mM NaCl), respectively. The result of analysis of variance showed that salt stress caused decrease of antioxidant properties studied. However, the simple interaction of titanium and salt stress indicated the increase trend. The mean comparison of chlorophyll contents (a, b and total), carotenoid and anthocyanin increased significantly by increase of titanium dioxide, however, it was decreased by increase of NaCl levels. According to the obtained results, it could be suggested to use of nano particle of titanium dioxide in order to improve the various traits such as morphological, physiological and biochemical under abiotic stresses in plants.