ﺗﻌﯿﯿﻦ اﻟﮕﻮي ﺑﺎﻧﺪي زﯾﺮواﺣﺪﻫﺎي ﮔﻠﻮﺗﻨﯿﻦ ﺑﺎ وزن ﻣﻮﻟﮑﻮﻟﯽ ﭘﺎﯾﯿﻦ LMW-Gs ﺑﺎ اﺳﺘﻔﺎده از ﻧﺸﺎﻧﮕﺮﻫـﺎي ﻣﺒـﺘﻨﯽ ﺑﺮ ﭘﺮوﺗﺌﯿﻦ در ژﻧﻮﺗﯿﭗﻫﺎي اﯾﺮاﻧﯽ ﮔﻨﺪم دوروم

Determination of the Banding Pattern of Low Molecular Weight Glutenin Subunits (LMW-Gs) Using Protein-based Markers in Iranian Durum Wheat Landraces

ﺗﺤﻘﯿﻖ ﺣﺎﺿﺮ ﺑﻪ ﻣﻨﻈﻮر ارزﯾﺎﺑﯽ ﮐﯿﻔﯿﺖ ﭘﺮوﺗﺌﯿﻨﯽ ژﻧﻮﺗﯿﭗﻫﺎي ﮔﻨﺪم دوروم ﺑﻮﻣﯽ اﯾﺮان از ﻟﺤﺎظ زﯾﺮواﺣﺪﻫﺎي ﮔﻠﻮﺗﻨﯿﻦ ﺑﺎ وزن ﻣﻮﻟﮑﻮﻟﯽ ﭘﺎﯾﯿﻦ )L(MW-Gs ﺑﺎ اﺳﺘﻔﺎده از SDS-PAGE اﺟﺮا ﺷ.ﺪ 79 ژﻧﻮﺗﯿﭗ ﺑﻮﻣﯽ ﮔﻨﺪم دوروم ﺟﻤﻊ آوري ﺷﺪه از ﻧﻘﺎط ﻣﺨﺘﻠﻒ اﯾﺮان ﺑﻪ ﻫﻤﺮاه دو رﻗﻢ ﺗﺠﺎرﺗﯽ دﻧﺎو ﮐﻮﻫﺮﻧﮓو 6 ژﻧﻮﺗﯿﭗ اﺳﺘﺎﻧﺪارد ﺣﺎوي زﯾﺮواﺣﺪﻫﺎي ﺷﻨﺎﺧﺘﻪ ﺷﺪه در ﻃﯽ ﺳﺎلﻫﺎي 1392-94 ارزﯾﺎﺑﯽ ﺷﺪﻧ.ﺪ ﺑﺮ اﺳﺎس اﻟﮕﻮي ﺣﺮﮐﺘﯽ ﻣﺘﻔﺎوت ﺑﺎﻧﺪﻫﺎي ﭘﺮوﺗﺌﯿﻦ در ژلﻫﺎي اﮐﺮﯾﻞآﻣﯿﺪ در ﻣﮑﺎنﻫﺎي ژﻧﯽ Glu-A3، Glu-B3و Glu-B2 ﺑﻪ ﺗﺮﺗﯿﺐ ،9 20و 3 آﻟﻞ ﻣﺨﺘﻠﻒ ﺷﻨﺎﺳﺎﯾﯽ ﺷﺪﻧ.ﺪ ﺗﻌﺪاد ﺑﺎﻻي آﻟﻞﻫﺎي ﺷﻨـﺎﺧﺘﻪ ﺷﺪه ﺑﻮﯾﮋه در ﻣﮑﺎنﻫﺎي ژﻧﯽ Glu-A3 و Glu-B3 ﺗﻨﻮع ﺑﺎﻻي ژﻧﺘﯿﮑﯽ ﺑﺮاي LMW-Gs را در ژﻧﻮﺗﯿﭗﻫﺎي ﮔﻨﺪم دوروم ﺑﻮﻣﯽ ﻧﺸﺎن ﻣﯽدﻫﺪ.

The present study was conducted to evaluate the protein quality of Iranian durum wheat landraces in terms of low molecular weight glutenin subunits (LMW-Gs) using SDS-PAGE. A panel of 79 Iranian durum wheat landraces collected from different parts of Iran along with two commercial cultivars i.e. Dena and Koohrang and 6 standard genotypes containing known LMW-Gs subunits were evaluated during 2013- 2015. Based on the different motility patterns of the protein bands in the acrylamide gels at the Glu-A3, Glu-B3 and Glu-B2 loci, 9, 20 and 3 different alleles were identified, respectively. The high number of alleles, especially in the Glu-A3 and Glu-B3 loci, shows high genetic variation for LMW-Gs in native durum wheat landraces