مطالعه in silico ناحيه پروموتري همولوگ هاي بتاآميرين-11-اكسيداز (CYP88D6) شيرين بيان(Glycyrrhiza glabra) در گياهان خانواده فاباسه

In silico analysis of promoter region in homologs of Beta-amyrin 11-oxidase of Glycyrrhiza glabra in Fabaceae family

ﺷﯿﺮﯾﻦﺑﯿﺎن از ﺑﺎ ارزشﺗﺮﯾﻦ ﮔﯿﺎﻫﺎن داروﯾﯽ ﺑﻮﻣﯽ اﯾﺮان اﺳﺖ ﮐﻪ داراي ﻣﺘﺎﺑﻮﻟﯿﺖﻫﺎي ﺛﺎﻧﻮﯾﻪي ﻣﺘﻌﺪدي از ﮔﺮوه ﺗﺮيﺗﺮﭘﻨﻮﺋﯿﺪﻫﺎ اﺳﺖ. ﮔﻠﯿﺴﯿﺮﯾﺰﯾﻦ ﻣﻬﻢ ﺗﺮﯾﻦ ﻣﺘﺎﺑﻮﻟﯿﺖ ﺛﺎﻧﻮﯾﻪ ي اﯾﻦ ﮔﯿﺎه اﺳﺖ. ﯾﮑﯽ از آﻧﺰﯾﻢﻫﺎي ﻣﻬﻢ در ﻣﺴﯿﺮ ﺑﯿﻮﺳﻨﺘﺰ ﮔﻠﯿﺴﯿﺮﯾﺰﯾﻦ، آﻧﺰﯾﻢ ﺑﺘﺎآﻣﯿﺮﯾﻦ11-اﮐﺴﯿﺪاز ﻣﯽﺑﺎﺷﺪ. در اﯾﻦ ﻣﻄﺎﻟﻌﻪ، ﻧﺎﺣﯿﻪي ﭘﺮوﻣﻮﺗﺮي ژن ﮐﺪ ﮐﻨﻨﺪه ﻫﻤﻮﻟﻮگﻫﺎي اﯾﻦ آﻧﺰﯾﻢ ﻣﻬﻢ در ﺗﻌﺪادي از ﮔﯿﺎﻫﺎن ﺧﺎﻧﻮادهي ﻓﺎﺑﺎﺳﻪ و ﺳﺎﯾﺮ ﺧﺎﻧﻮادهﻫﺎي ﮔﯿﺎﻫﯽ ﺑﺮ اﺳﺎس ﻣﻄﺎﻟﻌﺎت in silico ارزﯾﺎﺑﯽ ﺷﺪ. اﺑﺘﺪا 2000 ﺟﻔﺖ ﺑﺎز ﺑﺎﻻدﺳﺖ ﮐﺪون ﺷﺮوع ژن ﺑﺘﺎآﻣﯿﺮﯾﻦ11-اﮐﺴﯿﺪاز در ﮔﯿﺎﻫﺎن ﻫﺪف از ﺳﺎﯾﺖ ﻓﯿﺘﻮزوم درﯾﺎﻓﺖ و ﺳﭙﺲ ﺑﺎ اﺳﺘﻔﺎده از ﺳﺮور PlantCARE ﻣﻮرد آﻧﺎﻟﯿﺰ ﻗﺮار ﮔﺮﻓﺘﻨ.ﺪ ﻧﺘﺎﯾﺞ ﻧﺸﺎن داد ﻋﻨﺎﺻﺮ ﺗﻨﻈﯿﻤﯽ ﻣﺘﻌﺪدي در اﯾﻦ ﻧﺎﺣﯿﻪ ﭘﺮوﻣﻮﺗﺮي وﺟﻮد دارﻧﺪ ﮐﻪ در ﺗﻨﻈﯿﻢ ﺑﯿﺎن ژن اﻫﻤﯿﺖ دارﻧ.ﺪ ﺗﻌﺪادي از اﯾﻦ ﻋﻨﺎﺻﺮ ﺗﻨﻈﯿﻤﯽ ﻫﻤﭽﻮن P-box ﭘﺎﺳﺨﮕﻮ ﺑﻪ ﻓﯿﺘﻮﻫﻮرﻣﻮن ﺟﯿﺒﺮﻟﯿﻦ ﻣﯽﺑﺎﺷﻨ.ﺪ ﻋﻨﺎﺻﺮ ﺗﻨﻈﯿﻤﯽ ﺑﺎ ﻣﻮﺗﯿﻒ CGTCA ﺳﺒﺐ ﺑﯿﺎن ژن ﺗﺤﺖ اﺛﺮ ﻣﺘﯿﻞ ﺟﺎﺳﻤﻮﻧﺎت ﻣﯽﺷﻮد و MBS ﺑﯿﺎن ژن را در ﺷﺮاﯾﻂ ﺗﻨﺶ ﺧﺸﮑﯽ ﺗﻨﻈﯿﻢ ﻣﯽ ﮐﻨﻨﺪ. ﺑﺎ ﺗﻮﺟﻪ ﺑﻪ ﺷﻨﺎﺳﺎﯾﯽ ﻋﻨﺎﺻﺮ ﭘﺎﺳﺨﮕﻮ ﺑﻪ ﺑﺮﺧﯽ ﺗﻨﺶ ﻫﺎ از ﻗﺒﯿﻞ ﺧﺸﮑﯽ در ﻧﺎﺣﯿﻪ ﭘﺮوﻣﻮﺗﻮري اﯾﻦ ژن ﭘﯿﺸﻨﻬﺎد ﻣﯽ ﺷﻮد ﺑﺮاي اﻓﺰاﯾﺶ اﺣﺘﻤﺎﻟﯽ ﺑﯿﻮﺳﻨﺘﺰ ﮔﻠﯿﺴﯿﺮزﯾﻦ در اﯾﻦ ﮔﯿﺎه از اﻋﻤﺎل ﺗﻨﺶ ﻫﺎي ﻏﯿﺮ زﯾﺴﺘﯽ ﻫﻤﭽﻮن ﺧﺸﮑﯽ اﺳﺘﻔﺎده ﺷﻮد.

Liquorice is one of the most valuable native medicinal plants of Iran with numerous secondary metabolites of the triterpenoids group. Glycyrrhizin is a key secondary metabolite of this plant. One of the important enzymes in the glycyrrhizin biosynthesis pathway is Beta-amyrin 11-oxidase enzyme. In this study, the promoter region of the gene encoding homologs of this imperative enzyme was evaluated in some of Fabaceae family and other plant species based in silico analysis. First, 2000 bp upstream of the start codon of the beta-amyrin 11-oxidase gene in the target plants were obtained from the Phytosome site and then analyzed using the PlantCARE server. The results showed that there are several regulatory elements in this region that are important in regulating gene expression. A number of their regulatory elements were recognized including P-box affecting gene expression under gibberellin conditions, CGTCA-motif causing gene expression affected by methyl jasmonate and MBS influencing gene expression in drought.