Docetaxcel loaded polymeric micelles for treatment of breast cancer

Aim and Background: Docetaxel is a potent anticancer drug used to treat breast, and advanced non-small cell lung cancer. The marketed form of DTX (Taxotere® ) were often subjected to hypersensitivity after administration. In order to avoid these disadvantages more study have been developed. The amphiphilic pDMA-b-pPFPA used to load DTX due to its micelle formation. The physicochemical characteristics of DTX-loaded PMs such as size, zeta potential, CMC, the release study and cytotoxicity were investigated. Methods: Polymeric micelles were prepared by film method. The polymer and DTX solution were mixed and evaporated to obtain DTX/copolymer matrix. HEPES-sucrose (10mM) were used to hydrate. To remove the no-encapsulation drug centrifuged for in 14000 rpm for 10 min. finally, encapsulation efficacy was determined by HPLC method. MTT test used for evaluate the cytotoxicity. Results and discussion: DTX-PM were found to have size 38.50 nm and the zeta potential of −2.15 mV. DTX was well incorporated into PM with EE% 80. The DTX-PM released approximately 65% DTX during 48h. Cytotoxicity test against4T1cell showed that DTX-micelles had better in vitro cytotoxicity compared taxotere as control. Micelles internalized together with the entrapped DTX in the cytoplasm, probably via endocytic mechanism led improved cytotoxicity compared free drug. Conclusion: DTX well incorporated into Polymeric micelles with spherical shape showed that DTX-micelles had better in vitro cytotoxicity than taxotere