The Study of Quercetin-loaded Ca1-xMnxFe2O4 Nanocarriers for Anti-Cancer Activity on Breast Cancer Cells

Aim and Background: In this study, Ca1-xMnxFe2O4 spinel ferrite nanocarriers (NCs) were synthesized for use on drug delivery systems (DDS) to deliver Quercetin (Que). Methods: Ca1-xMnxFe2O4 (0 x 1) NCs were synthesized using the thermal treatment. Then the Que was loaded on NCs by acetone using the precipitation. To measure the percentage of drug loading (DL), 1 mg of Que loaded with dried NCs is dissolved in acetone. Then, the amount of Que is measured using spectroscopy (370 nm). The cytotoxicity of NCs is evaluated using the MTT method. Results and discussion: The FT-IR spectrum of NCs and Que-loaded on NCs provided more information about the effectiveness of Que ligand grafting on the surface nanoparticle that showed successful functionalization of the Que on the NCs surface. The Que Drug loading study has been performed that showed good results and it may be due to the fewer structural defects of NCs with more space for the guest molecules. The cytotoxicity of Que-loaded on NCs against cancer cells was investigated differently for each sample at different concentrations. The percentage of MCF-7 cancer cell destruction was increased with increasing the NCs from 1 to 60 µg/ml, which enhanced permeability and retention (EPR) can lead to easy penetration of Que from NCs into MCF-7 cells. Conclusion: Que-loaded on Ca1-xMnxFe2O4 NCs could increase significantly the anti-cancer activity of MCF-7 cells and are suitable samples to use in the DDS.