Genotype and phenotype relationships of C/T (rs 1800668) and 198 Pro/Leu polymorphisms of glutathione peroxidase 1(GPx 1) in stenosed coronary arteries; The C variant is related to GPx 1 activity

Genotype and phenotype relationships of C/T (rs 1800668) and 198 Pro/Leu polymorphisms of glutathione peroxidase 1(GPx 1) in stenosed coronary arteries; The C variant is related to GPx 1 activity

In this study, we investigated Ox-LDL level and GPx1 activity on the extent of stenosis in coronary arteries. In addition, the role of important variants of GPx1 gene, kinetic and prediction studies evaluated for our better conception of gene changes in GPx1function. One hundred fifty subjects were recruited from who underwent coronary angiography on the study design, and were subdivided on the extent of stenosis in coronary arteries (controls; n=55: Stenosis <5% and Patients; n=95: SVD, 2VD, 3VD: Stenosis >50%). The Ox-LDL level and GPx1 activity were measured using ELISA and spectrophotometric methods, respectively. rs 1050450 (198 Pro/Leu) and rs 1800668 (C/T) polymorphisms were identified using RFLP-PCR method. After GPx1 purification with salting out and gel filteration chromatography (Sephacryl S-300) techniques, the Km changes studied for homozygotes (rs 1800668 CC and TT). We found no significant associations for the Ox-LDL level (P=0.2) and GPx1 activity (P=0.96) and rs 1050450 genotype distribution (P=0.79) between groups. Furthermore, we observed a genotype and phenotype relationship for rs 1800668 site located in the GPx1 promoter region. We concluded that the oxidative theory has not a substantial role in development of stenosis of coronary arteries in our patients