تأثير هشت هفته تمرين استقامتي با و بدون عصاره ي پسته ي وحشي (بنه) بر بيان ژن ABCG5 كبد موش هاي صحرايي ماده

حامل های ABC خانواده بزرگی از پروتئین حامل غشایی اند كه با استفاده از انرژی هیدرولیز ATP طیف گسترده ای از سوبستراها را در غشا های بیولوژیكی جابجا كرده و در فرآیند انتقال معكوس كلسترول نقش بسزایی دارند. پروتئین ABCG5 یكی از اعضای این خانواده بزرگ است كه نقش مهمی در این فرآیند ایفا می كند. پسته ی وحشی( بنه) گیاهی از خانواده آناكاردیاسه دارای خواص ضد التهابی و ضداكسایشی است. هدف از پژوهش حاضر بررسی اثر هشت هفته تمرین استقامتی با و بدون عصاره ی بنه بر میزان بیان ژن ABCG5 كبد است. این پروتئین ها در حمل و نقل مواد مختلفی مثل اسیدهای آمینه، ذرات ارگانیك، پیتیدها، قندها، لیپوپلی ساكلریدها، بسیاری از داروها و پروتئین ها بسیار ضروری و مهم هستند. نشان داده شده است كه ABCG5 انتقال دهنده اصلی ترشح كلسترول صفراوی در نظر گرفته می ود. مطالعه ی حاضر قصد دارد نقش دو عامل ورزش و مكمل غذایی بنه را در میزان بیان ژن ABCG5 بافت كبد را بررسی كند.

Introduction: ABC transporters comprise a large family of transmembrane proteins that use the energy of ATP hydrolysis to translocate a wide variety of substrates across biological membranes and have a significant role in the process of reverse cholesterol transport. ABCG5 protein is a member of the family that plays a crucial role in this process. Pistaciaatlantica (Baneh) are plants of Anacardiaceae family that has anti-oxidative compounds and reduces free radicals. The current study was conducted to investigate the effect of treadmill running with or without Baneh extraction on the relative gene expression of ABCG5of female rat in liver tissue. Methods: In this study twenty wistar rats (six to eight weeks old, 125-135 g weight) were used. Animals were randomly assigned into training (n = 10) and control (n = 10) groups and further divided into saline-control, saline-training, Baneh-control and Baneh-training. Training groups was given exercise on a motor-driven treadmill at 25 m/min (0% grade) for 60 min/day, 5 days/week for eight weeks. Subjects were fed as the oral with Baneh extraction and saline for four week. After PCR procedure, ABCG5 gene expression was detect by Real-time PCR method. Results: data analysis revealed a significant difference in liver ABCG5 mRAN relative gene expression between groups (P<0.001). Using a suitable following post hoc test has showed that ABCG5 fold change was higher in saline-training group when compared with other groups at the end of program. On the other hand, a lower and higher ABCG5 fold changes were found in Baneh and trained groups, respectively. Conclusion: This research showed an increase in ABCG5 gene expression in training group. ABCG5 gene expression also significantly decreased in the saline group was observed, which it is possible to reduce the ABC transporter is due to the high fatty acid of baneh.