Takayuki Sugiura، نويسنده , , Shinji Nakane، نويسنده , , Seishi Kishimoto، نويسنده , , Keizo Waku، نويسنده , , Yasuko Yoshioka، نويسنده , , Akira Tokumura، نويسنده , , Donald J. Hanahan، نويسنده ,

1601492

Occurrence of lysophosphatidic acid and its alkyl ether-linked analog in rat brain and comparison of their biological activities toward cultured neural cells

11715

Rat brain was found to contain substantial amounts of potent bioactive lipids lysophosphatidic acid (acyl LPA) (3.73 nmol/g tissue) and lysoplasmanic acid (alkyl LPA) (0.44 nmol/g tissue). The presence of alkyl LPA was confirmed by mild alkaline hydrolysis analysis and by gas chromatography/mass spectrometry analysis of the trimethylsilyl derivative. This is the first clear evidence of the occurrence of an alkyl LPA in nature. The predominant molecular species of acyl LPA are 18:1-, 18:0- and 16:0-containing species (46.9, 22.5 and 18.8%, respectively). A significant amount of a 20:4-containing species (7.2%) was also detected in the acyl LPA fraction. We also confirmed that rat brain alkyl LPA consists of 16:0-, 18:0- and 18:1-containing species. Noticeably, either acyl or alkyl LPA is capable of stimulating neuroblastoma×glioma hybrid NG108-15 cells to elicit a Ca2+ transient, the potencies being almost the same. Both acyl and alkyl LPAs also induce cell rounding upon addition to the cells. These results suggest that acyl and alkyl LPAs play important physiological roles as intercellular signaling molecules as well as the roles as metabolic intermediates in the nervous system.

194

Lysophosphatidic acid , Alkyl ether-linked lysophospholipid , platelet-activating factor , GC/MS analysis , Brain , rat , Ca2? transient

Studia Iranica

204

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