Sravan Kumar Goparaju، نويسنده , , Yuko Kurahashi، نويسنده , , Hiroshi Suzuki، نويسنده , , Natsuo Ueda، نويسنده , , Shozo Yamamoto، نويسنده ,

1601509

Mouse osteoblastic cells MC3T3-E1 produced prostaglandin E2 via the reaction of cyclooxygenase-2 enzyme induced by tumor necrosis factor α (TNFα). Originally, the mRNA level for prostaglandin I2 receptor (IP) was low in the cells. However, the addition of

11732

Anandamide (arachidonoylethanolamide) is an endogenous ligand for cannabinoid receptors, and its cannabimimetic activities are lost when the compound is hydrolyzed to arachidonic acid and ethanolamine by an enzyme referred to as anandamide amidohydrolase. We cloned a cDNA for the enzyme of porcine brain, and the cDNA encoded a protein of 579 amino acids with a molecular mass of 62.9 kDa. The amino acid sequence was 81, 80 and 85% identical with the enzymes previously cloned from the liver of rat, mouse, and human, respectively. When the enzyme protein was overexpressed in COS-7 cells, the particulate fraction of the cells showed an anandamide hydrolyzing activity and also catalyzed the reverse reaction synthesizing anandamide from arachidonic acid and ethanolamine both with a specific activity of 0.2–0.3 μmol/min/mg protein at 37°C. The brain enzyme exhibited a wide substrate specificity hydrolyzing oleamide, 2-arachidonoylglycerol, and methyl arachidonate. The point mutation of Ser-217, Asp-237, Ser-241, or Cys-249 completely abolished the hydrolyses of all the above-mentioned substrates as well as the synthesis of anandamide in the reverse reaction.

77

Anandamide , cannabinoid , Fatty acid amide hydrolase , Amidohydrolase , brain , (Pig)

Studia Iranica

84

84