Author/Authors
Yan Chen، نويسنده , , William E. Zackert، نويسنده , , L. Jackson RobertsII، نويسنده , , Jason D. Morrow، نويسنده ,
DocumentNumber
1601353
Title Of Article
Evidence for the formation of a novel cyclopentenone isoprostane, 15-A2t-isoprostane (8-iso-prostaglandin A2) in vivo
شماره ركورد
11810
Latin Abstract
A2/J2-Isoprostanes (IsoPs) are prostaglandin (PG) A2/J2-like compounds that are produced in vivo as dehydration products of D2/E2-IsoPs. One A2-IsoP that should be formed is 15-A2t-IsoP (8-iso-PGA2). Analogous to cyclopentenone PGs, 15-A2t-IsoP readily undergoes nucleophilic addition to various biomolecules suggesting the compound is capable of exerting potent bioactivity. However, proof that it is definitively formed in vivo is lacking. Evidence is now presented that 15-A2t-IsoP, in fact, is generated in vivo by demonstrating that an endogenous A2-IsoP with a retention time on capillary GC identical with that 15-A2t-IsoP co-chromatographs through four high resolving HPLC purification procedures with authentic radiolabeled 15-A2t-IsoP.
From Page
550
NaturalLanguageKeyword
prostaglandin , Eicosanoid , cyclopentenone , Lipid peroxidation , isoprostane
JournalTitle
Studia Iranica
To Page
556
To Page
556
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