Author/Authors

Koichi Sakurada، نويسنده , , Hirotaro Iwase، نويسنده , , Takehiko Takatori، نويسنده , , Masataka Nagao، نويسنده , , Makoto Nakajima، نويسنده , , Hitoshi Niijima، نويسنده , , Yukimasa Matsuda، نويسنده , , Masahiko Kobayashi، نويسنده ,

DocumentNumber

1601384

Title Of Article

Identification of cis-9,10-methylenehexadecanoic acid in submitochondrial particles of bovine heart

شماره ركورد

11841

Latin Abstract

Submitochondrial particles of bovine heart were hydrolyzed by phospholipase A2 and the products were analyzed by liquid chromatography electrospray ionization-mass spectrometry. We found a fatty acid with a molecular mass of 268 Da and a retention time longer than that of linoleic acid. Next, we synthesized organically cis-9,10-methylenehexadecanoic acid, which has a molecular mass similar to that of the extracted fatty acid, and characterized its high performance liquid chromatography and gas chromatography-mass spectrometry profiles. Using these data we were able to identify endogenous cis-9,10-methylenehexadecanoic acid in rat and human heart and liver tissues that had been hydrolyzed by phospholipase A2. This fatty acid was not detected in tissue extracts that had not been hydrolyzed by phospholipase A2. Similar amounts of cis-9,10-methylenehexadecanoic acid were measured in tissue extracts after total hydrolysis. These results suggest that cis-9,10-methylenehexadecanoic acid is a fatty acid component, in the sn-2 position, of phospholipids in some mammalian tissue.

From Page

214

NaturalLanguageKeyword

Cyclopropane fatty acid , Bovine , human , mitochondria , phospholipase A2

JournalTitle

Studia Iranica

To Page

222

To Page

222