Author/Authors :
Didier Rotticci، نويسنده , , Torbj?rn Norin، نويسنده , , Karl Hult، نويسنده , , Mats Martinelle، نويسنده ,
Title Of Article :
An active-site titration method for lipases
Latin Abstract :
A method for active-site titration of lipases has been developed based on irreversible inhibition by methyl p-nitrophenyl n-hexylphosphonate. This method was applied to five lipases displaying from minor to pronounced interfacial activation. Soluble and immobilized lipases were successfully titrated in aqueous media. A low concentration of sodium dodecyl sulfate was needed for lipases displaying pronounced interfacial activation. The carrier of some of the immobilized preparations adsorbed part of the produced p-nitrophenolate. This problem could be solved by extracting the p-nitrophenolate after inhibition. The method was extended to apolar organic solvents in the case of immobilized lipase preparations.
NaturalLanguageKeyword :
aqueous medium , Organic medium , Concentration , Interfacial activation , inhibitor , detergent , Immobilized lipase
JournalTitle :
Studia Iranica
