1601660

11976

Cholesterol sulfate (CS) and sulfatides in the epithelium of the digestive tract were found in the 1000×g supernatants of digestive fluid, particularly in gastric juices containing the duodenal contents and bile acids, there being 14–131 μg of CS and 3–54 μg of sulfatides per mg of protein in the fluid, respectively. CS and sulfatides dissolved in detergents including bile acids inactivated pancreatic trypsin to the same level as by DMSO-solubilized sulfated lipids at 37°C. Similarly, pancreatic DNase I was inhibited by CS solubilized with DMSO or bile acids, but not by sulfatides or other membrane lipids at 37°C. Both the sulfate group and the hydrophobic side chain of CS were indispensable structures for the inhibition of DNase I. Also, the optimum molar ratio of bile acids to CS was important for expression of the inhibitory activity of CS toward DNase I, it being 0.18 of the optimum ratio for sodium taurocholate, and the molar ratio of CS to DNase I for complete inhibition was 342:1. Thus, CS was shown to play a role as an epithelial inhibitor of DNase I in concert with bile acids.

268

Digestive tract , Micelle , Sodium dodecyl sulfate , Sulfatide , Noncompetitive inhibition , Trypsin , glycolipid

Studia Iranica

274

274