Author/Authors :
Mark Abramovitz، نويسنده , , Mohammed Adam، نويسنده , , Yves Boie، نويسنده , , Marie-Claude Carrière، نويسنده , , Danielle Denis، نويسنده , , Claude Godbout، نويسنده , , Sonia Lamontagne، نويسنده , , Chantal Rochette، نويسنده , , Nicole Sawyer، نويسنده , , Nathalie M. Tremblay، نويسنده , , Michel Belley، نويسنده , , Michel Gallant، نويسنده , , Claude Dufresne، نويسنده , , Yves Gareau، نويسنده , , Rejean Ruel، نويسنده , , Helene Jut، نويسنده ,
Latin Abstract :
Stable cell lines that individually express the eight known human prostanoid receptors (EP1, EP2, EP3, EP4, DP, FP, IP and TP) have been established using human embryonic kidney (HEK) 293(EBNA) cells. These recombinant cell lines have been employed in radioligand binding assays to determine the equilibrium inhibitor constants of known prostanoid receptor ligands at these eight receptors. This has allowed, for the first time, an assessment of the affinity and selectivity of several novel compounds at the individual human prostanoid receptors. This information should facilitate interpretation of pharmacological studies that employ these ligands as tools to study human tissues and cell lines and should, therefore, result in a greater understanding of prostanoid receptor biology.
NaturalLanguageKeyword :
Prostanoid receptor , prostaglandin , Radioligand binding , Prostaglandin analog , recombinant , Human embryonic kidneycell line
