Author/Authors :
Daniela Peruzzi، نويسنده , , Giuseppe Calabrese، نويسنده , , Irene Faenza، نويسنده , , Lucia Manzoli، نويسنده , , Alessandro Matteucci، نويسنده , , Fernando Gianfrancesco، نويسنده , , Anna Maria Billi، نويسنده , , Liborio Stuppia، نويسنده , , Giandomenico Palka، نويسنده , , Lucio Cocco، نويسنده ,
Latin Abstract :
Members of phosphoinositide-specific phospholipase C (PLC) families are central intermediary in signal transduction in response to the occupancy of receptors by many growth factors. Among PLC isoforms, the type β1 is of particular interest because of its reported nuclear localisation in addition to its presence at the plasma membrane. It has been previously shown that both the stimulation and the inhibition of the nuclear PLCβ1 under different stimuli implicate PLCβ1 as an important enzyme for mitogen-activated cell growth as well as for murine erythroleukaemia cell differentiation. The above findings hinting at a direct involvement of PLCβ1 in controlling the cell cycle in rodent cells, and the previously reported mapping of its gene in rat chromosome band 3q35–36, a region frequently rearranged in rat tumours induced by chemical carcinogenesis, prompted us to identify its human homologue. By screening a human foetal brain cDNA library with the rat PLCβ1 cDNA probe, we have identified a clone homologous to a sequence in gene bank called KIAA 0581, which encodes a large part of the human PLCβ1. By using this human cDNA in fluorescence in situ hybridisation on human metaphases, it has been possible to map human PLCβ1 on chromosome 20p12, confirming the synteny between rat chromosome 3 and human chromosome 20 and providing a novel locus of homology between bands q35–36 in rat and p12 in man. Since band 20p12 has been recently reported amplified and/or deleted in several solid tumours, the identification and chromosome mapping of human PLCβ1 could pave the way for further investigations on the role exerted both in normal human cells and in human tumours by PLCβ1, which has been shown to behave as a key signalling intermediate in the control of the cell cycle.
NaturalLanguageKeyword :
chromosome , gene , Human phospholipase CL1 , Nucleus
