Ping Zheng، نويسنده , , Andrew Horwitz، نويسنده , , Christine A. Waelde، نويسنده , , Jonathan D. Smith، نويسنده ,

1601826

Stably transfected ABCA1 antisense cell line has decreased ABCA1 mRNA and cAMP-induced cholesterol efflux to apolipoprotein AI and HDL

12260

Using a sensitive real time fluorescent PCR assay, ABCA1 mRNA levels were induced by 50–70-fold following 8Br-cAMP treatment of the RAW264 murine macrophage cell line, concomitant with the induction of cholesterol efflux to apoAI and HDL. A stably transfected ABCA1 antisense cDNA cell line was created, which led to 50–70% reductions in ABCA1 mRNA levels in basal and 8Br-cAMP-treated cells, and diminished to the same extent the 8Br-cAMP-mediated efflux of cholesterol to apolipoprotein AI and HDL. These data demonstrate that ABCA1 is necessary for the cAMP-induced lipid efflux to both apoAI and HDL.

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Cholesterol e¥ux , Real time PCR , apolipoprotein AI , HDL , Antisense cDNA , ABCA1

Studia Iranica

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