مرکز منطقه ای اطلاع رساني علوم و فناوري - Hot saline irrigation as an alternative local adjuvant therapy in local aggressive bone tumors

Latin Abstract :

Objectives: This study aimed to develop an alternative thermoin- activation method for biological inactivation of microscopic conta¬mination on the cavity wall following curettage of local aggressive tumors. Hot saline irrigation was applied at various temperatures to bone cavity, with or without tourniquet on the extremity and tem¬perature changes and local effects were investigated in vivo. Methods: Bone cavities 12 cm3 in size were created in the condylar regions of the hind legs in nine female adult sheep. The cavities were irrigated by hot saline solution at 60, 70, 75, 80, or 100 °C, with and without the presence of a tourniquet, and the temperatures 1, 2, 3, and 10 mm distant to the cavity wall were recorded. The animals were sacrificed postoperatively at 2 days, 3 and 6 weeks, and 3 and 7 months for histologic studies. Results: The mean body temperature and temperature inside the bone prior to surgery were measured as 38.1 °C and 27.34 °C, respectively. Tourniquet application caused a mean decrease of 1.5 °C (range 1-2 °C) in bone temperature. The highest temper¬atures measured below 80 °C and at 80 °C were 55.5 °C and 62.5 °C in the cavity, and 40.5 °C and 42.5 °C in the bone, respectively. At 100 °C application, the color of the bone togeth¬er with the surrounding soft tissue and muscle tissue turned to yellow-brown-black, and the animal died on the second postop¬erative day. At temperatures <80 °C, the color of the bone and surrounding tissues appeared normal and no early complications were encountered. Histologic studies showed no bone marrow or bone necrosis at 60 °C, only bone marrow necrosis at 70 °C and 75 °C, and in addition to bone marrow necrosis, bone necro¬sis at 80 °C. At these temperatures no evidence for soft tissue necrosis were observed. Conclusion: Our results show that, in order to achieve bone necrosis at 1-mm and 2-mm distances from the cavity wall, the optimum temperature for hot saline irrigations applied to the bone cavity is 80 °C.