Author/Authors :
Boğa, Mehmet Dicle University - Faculty of Pharmacy - Department of Pharmaceutical Technology, Turkey , Ertaş, Abdulselam Dicle University - Faculty of Pharmacy - Department of Pharmacognosy, Turkey , Yeşil, Yeter Istanbul University - faculty of Pharmacy - Department of Pharmaceutical Botany, Turkey , Haşimi, Nesrin Babnan University - School of Health - Department of Nutrition and Dietetics, Turkey , Yılmaz, Mustafa Abdullah Dicle University - Research and Application of Science and Technology Center (DÜBTAM), Turkey , Özaslan, Cumali Dicle University - Faculty of Agriculture - Department of Plant Protection, Turkey
Title Of Article :
Phytochemical analysis and antioxidant and anticholinesterase activities of Pulicaria dysenterica from Turkey
Abstract :
This study is the first antioxidant and anticholinesterase activity report on phytochemical analysis and biological activity Pulicaria dysenterica. The essential oil analysis and fatty acids of the petroleum ether extract of P. dysenterica was determined by GC/MS analysis. The major essential oil was identified as terpinolen (32.6%). Also thirteen fatty acids were identified, constituting 99.0% of the petroleum ether extract. Main components were identified as palmitic acid (34.2%), linoleic (16.4%) and stearic acid (15.7%). β-carotene-linoleic acid test system, DPPH free radical scavenging assay, ABTS cation radical scavenging assay and cupric reducing antioxidant capacity assays were used for studying the antioxidant activity of the petroleum ether, acetone, methanol and water extracts of this plant. The water extract exhibited strong ABTS cation radical scavenging activity at 100 μg/mL. Ellman method was used to indicate the acetyl- and butyryl-cholinesterase inhibitory activities of the crude extracts. This is where, the petroleum ether extract exhibited 65.33% inhibitory activity against acetylcholinesterase at 200 μg/mL. The results showed that P. dysenterica is a potential source of ABTS cation radical scavenging products
NaturalLanguageKeyword :
Asteraceae , Pulicaria dysenterica , essential oil , fatty acid , antioxidant , anticholinesterase
JournalTitle :
dicle university journal of institute of natural and applied science
