Gürkan, Mert Çanakkale Onsekiz Mart University, Terzioğlu Campus - Faculty of Sciences and Arts - Department of Biology, Zoology Section, Turkey , Hayretdağ, Sibel Çanakkale Onsekiz Mart University, Terzioğlu Campus - Faculty of Sciences and Arts - Department of Biology, Zoology Section, Turkey , Yakın, Batuhan Yaman Çanakkale Onsekiz Mart University, Terzioğlu Campus - Faculty of Sciences and Arts - Department of Biology, Zoology Section, Turkey , Tok, Cemal Varol Çanakkale Onsekiz Mart University,Terzioğlu Campus - Faculty of Sciences and Arts - Department of Biology, Zoology Section, Turkey

A preliminary study on micronucleus analysis and nuclear anomalies in Pelophylax ridibundus (Pallas, 1771) (Amphibia: Anura) specimens collected around Vize (Kirklareli) and Ida Mountains (Çanakkale, Turkey)

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In this study, a micronucleus analysis was made on the erythrocytes of the Pelophylax ridibundus specimens collected around Vize (Kırklareli) and Ida Mountains (Yenice, Çanakkale), nuclear anomalies were detected. For this purpose, 9 (5♂♂, 4♀♀) P. ridibundus specimens were collected from Vize in April 2011 and 5 (3♂♂, 2♀♀) P. ridibundus specimens were collected from Ida Mountains in May 2011. The mean number of micronuclei was calculated as 6±4.17 and frequency as 0.3% in the specimens collected from Vize, while the mean number of micronuclei was calculated as 0.6±0.43 and frequency as 0.03% in the specimens collected from Ida Mountains. As a result of the statistical analyses, it was determined that there was a significant difference in the total number of micronuclei between both localities (p≤0.05). Some 4 types of nuclear anomalies, i.e. binucleate, notched, blebbed and small-lobed, were detected in the study. No difference in the total number of nuclear anomalies was detected between the specimens of Vize and Ida Mountains (p=0.31). According investigation to the results, Vize samples have a higher frequency of micronuclei associated with intensive agricultural activities used genotoxic exposure to pesticides.

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Pelophylax ridibundus , Micronucleus , Nuclear anomalies , Vize , Ida Mountains

Ege Journal Of Fisheries an‎d Aquatic Sciences

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