مرکز منطقه ای اطلاع رساني علوم و فناوري - The effects of dioctyl adipate (DOA) on liver and gill histology of Sparus aurata ( Sea bream)

Abstract :

Although it is used widely as a second plasticizer in the polymer manufacturing process, the ecotoxicological effects of dioctyl adipate (DOA) was not investigated in detail. The aim of this research was to determine the histopathological changes in the gills and liver of male sea bream, Sparus aurata exposed to 250, 500 and 750 ppm DOA for 15 days, with light microscopy. When compared with control, remarkable histopathological changes as vacuolation, dilatation of the central vein, and a striking fusion were observed in the gills of experimental fish. It was also noted hypertrophy and hyperplasia in the epithelial, pillar, mucoid and mitochondria-rich cells of the gills. The examining of livers from DOA-exposed specimens revealed a series of hepatocellular changes, including steatosis, necrosis and fibrosis. In addition to haemorrage, the parenchimatic sinusoids and the central veins of hepatopancreas were widened. The study demonstrated that the exposure to DOA results in multiple histological changes in both of the tissue investigated; moreover, all of the changes were associated with increasing concentration of the chemical that exposed. Consequently, it was emphasized that DOA needs to be taken into more consideration as being an aquatic ecotoxicant.